Y4645
Anti-phospho-YAP1 (pTyr357) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-YAP, Anti-YAP2, Anti-YAP65, Anti-Yes-associated protein 1, 65kDa
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
8
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.5 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using HEK-293 cells co-transfected with human YAP1 and c-Abl, western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and human c-Abl
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr357)
Gene Information
chicken ... YAP1(396171)
human ... YAP1(10413)
mouse ... Yap1(22601)
Related Categories
General description
Yes-associated protein 1 (YAP1) is a 65 kDa and a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with Src-family tyrosine kinase c-Yes. In addition to a SH3-binding motif, YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 binds to p73 through its WW domain and the PPPY motif of p73. This interaction is required for the ability of YAP1 to co-activate p73-responsive genes.
Immunogen
synthetic peptide corresponding to amino acids 351-362 (pTyr357) located in the C-terminal region of human YAP1. The sequence is identical in chicken YAP1 and highly conserved in mouse YAP1 (single amino acid substitution).
Application
Anti-phospho-YAP1 (pTyr357) antibody produced in rabbit has been used in western blotting and immunofluorescence.
Biochem/physiol Actions
Anti-phospho-YAP1 pTyr357 antibody is specific for human phospho-YAP1 pTyr357. The product may react with chicken and mouse phospho-YAP1 pTyr357. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.
Inhibition of Akt potentiates the nuclear re-localization of YAP1 to induce apoptosis by p73.
YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development
Anti-phospho-YAP1 pTyr357 antibody is specific for human phospho-YAP1 pTyr357. The product may react with chicken and mouse phospho-YAP1 pTyr357. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.
Anti-phospho-YAP1 pTyr357 antibody is specific for human phospho-YAP1 pTyr357. The product may react with chicken and mouse phospho-YAP1 pTyr357. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
常规特殊物品
常规特殊物品
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Conjugated Bile Acids Promote Invasive Growth of Esophageal Adenocarcinoma Cells and Cancer Stem Cell Expansion via Sphingosine 1-Phosphate Receptor 2--Mediated Yes-Associated Protein Activation
Liu R, et al.
The American Journal of Pathology, 188(9), 2042-2058 (2015)
The mammalian Hippo pathway: regulation and function of YAP1 and TAZ
Kodaka M, et al.
Cellular and Molecular Life Sciences, 72 (2015)
Akt Phosphorylates the Yes-Associated Protein, YAP, to Induce Interaction with 14-3-3 and Attenuation of p73-Mediated Apoptosis
Basu S, et al.
Molecular Cell, 11 (2003)
Akihiko Komuro et al.
The Journal of biological chemistry, 278(35), 33334-33341 (2003-06-17)
The ErbB-4 receptor protein-tyrosine kinase is proteolytically processed by membrane proteases in response to the ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation resulting in the cytoplasmic fragment translocating to the cell nucleus. The WW domain-containing co-transcriptional activator Yes-associated protein (YAP) associates physically with
Fernando D Camargo et al.
Current biology : CB, 17(23), 2054-2060 (2007-11-06)
The mechanisms that regulate mammalian organ size are poorly understood. It is unclear whether the pathways that control organ size also impinge on stem/progenitor cells. A highly expressed gene in stem cells is YAP1, the ortholog of Drosophila Yorkie, a
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| Y4645-100UL | 04061835667703 |
| Y4645-200UL | 04061838038333 |
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