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Astec® CHIROBIOTIC® V2 Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

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eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

Agency

suitable for USP L88

description

HPLC column

product line

Astec®

packaging

pkg of 1 ea

manufacturer/tradename

Astec®

parameter

0-45 °C temperature
241 bar pressure (3500 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

25 cm × 4.6 mm

matrix

High-purity silica gel particle platform
fully porous particle

matrix active group

vancomycin phase

particle size

5 μm

pore size

200 Å

operating pH range

3.5-7.0

separation technique

chiral

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General description

Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC® V in the polar ionic mode. The CHIROBIOTIC® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.

  • Bonded phase: Vancomycin
  • Operating pH range: 3.5 - 7.0
  • Particle diameter: 5, 10 or 16 μm
  • Pore size: 100 Å (CHIROBIOTIC® V) or 200 Å (CHIROBIOTIC® V2)

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature

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Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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Description
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Chromatography Liquid Chiral Separations
Xiao, T.L., et al.
Encyclopedia of Separation Science, 1-15 (2000)
New chemo-enzymatic approaches for the synthesis of (R)- and (S)-bufuralol
Nagy, Botond, et al.
Tetrahedron Asymmetry, 25 (18-19), 1316-1322 (2014)
Doris Frühauf et al.
Journal of chromatography. A, 1269, 242-254 (2012-10-30)
A sub-minute enantioselective normal phase high performance liquid chromatographic (HPLC) method for the analysis of a chiral precursor molecule employed frequently in folic acid syntheses was developed, optimized and successfully validated according to ICH-guidelines. It could be shown that ultra-high
Enantioselective and sensitive determination of carvedilol in human plasma using chiral stationary-phase column and reverse-phase liquid chromatography with tandem mass spectrometry
Jiang, Juanjuan, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 960, 92-97 (2014)
Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry
Liu, Ke, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 864 (1-2), 129-136 (2008)

Protocols

Efficient LC-MS separation of methamphetamine enantiomers in urine allows sensitive differentiation of illicit D-methamphetamine from over-the-counter L-methamphetamine.

Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine

In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine.

Related Content

Astec CHIROBIOTIC™ V2 Chiral HPLC Columns offered by Sigma-Aldrich. The polar organic and the polar ionic modes have become increasingly popular largely because of their ideal compatibility with MS detection and preparative LC.

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