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50879-U

Ascentis® Express 90 Å F5 (2 μm) HPLC Columns

L × I.D. 10 cm × 3 mm UHPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
10 cm × 3 mm
Particle size:
2 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
90 Å pore size
Matrix:
Fused-Core particle platform, superficially porous particle
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Product Name

Ascentis® Express F5, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 10 cm × 3 mm

material

stainless steel column

Quality Segment

agency

suitable for USP L43

product line

Ascentis®

feature

endcapped

analyte functional class(es)

antibiotics, explosives, fat soluble vitamins, sweeteners, water soluble vitamins

analyte chemical class(es)

PFAS, aliphatic amines, alkaloids, amino acids, aromatic amines, coloring compounds, nucleosides, phenols, small carbohydrates, steroids (cholesteryl esters)

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

1000 bar max. pressure (14500 psi), 60 °C temp. range

technique(s)

LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

10 cm × 3 mm

surface area

120 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

2 μm

pore size

90 Å pore size

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

General description

Ascentis® Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core® particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis® Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Having polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis® Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

UHPLC/MS Analysis of Mycotoxins on Ascentis® Express F5, 2 μm

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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