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Ascentis^® Express 90 Å F5 (2.7 μm) HPLC Columns

Name: Ascentis<SUP>&#174;</SUP> Express 90 Å F5 (2.7 μm) HPLC Columns
Brand: SUPELCO

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core PFP HPLC column

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About This Item

UNSPSC Code:

41115700

eCl@ss:

32110501

NACRES:

SB.52

L × i.d.:

15 cm × 4.6 mm

Particle size:

2.7 μm

Matrix active group:

PFP (pentafluorophenyl) phase

Pore size:

90 Å pore size

Matrix:

Fused-Core particle platform, superficially porous particle

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Properties

Product Name

Ascentis^® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Segment

100

agency

suitable for USP L43

product line

Ascentis^®

feature

endcapped

manufacturer/tradename

Ascentis^®

packaging

1 ea of

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

135 m²/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

Description

General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study demonstrates the use of high-performance liquid chromatography (HPLC) with ion trap time-of-flight mass spectrometry for the determination of lumacaftor in the presence of ivacaftor. The research identifies five novel degradation products, showcasing the advanced analytical capabilities of the Ascentis^® Express F5, 2.7 µm HPLC Column for complex mixtures and degradation studies (Özcan et al., 2023).

Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: This paper details the development of stability-indicating methods using LC-MS/MS and LC-DAD for the determination of tasimelteon. It highlights the robustness and sensitivity of these methods in identifying novel degradation products, with the Ascentis^® Express F5 column playing a crucial role in separation efficiency and stability analysis (Özcan et al., 2020).

Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review article discusses recent advancements in ultra-fast HPLC, focusing on new generation superficially porous silica columns, including the Ascentis^® Express F5, 2.7 µm HPLC Column. It provides insights into the enhanced performance, speed, and efficiency of these columns in high-throughput analytical applications (Ali et al., 2012).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)