59166
SUPELCOSIL™ LC-Si (5 µm) HPLC Columns
L × I.D. 30 cm × 4 mm, HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
30 cm × 4 mm
Particle size:
5 μm
Matrix active group:
Si, silica phase
Pore size:
120 Å pore size
Matrix:
silica gel, spherical particle platform
Product Name
SUPELCOSIL™ LC-Si HPLC Column, 5 μm particle size, L × I.D. 30 cm × 4 mm
agency
suitable for USP L3
Quality Segment
manufacturer/tradename
SUPELCOSIL™
parameter
≤70 °C temp. limit, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
30 cm × 4 mm
surface area
170 m2/g
matrix
silica gel, spherical particle platform
matrix active group
Si, silica phase
particle size
5 μm
pore size
120 Å pore size
pH range
2-7.5
application(s)
food and beverages
compatibility
mode of use normal phase HPLC
separation technique
hydrophilic interaction (HILIC), normal phase
General description
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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