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67459-U

BIOshell^™ A400 Protein C18 (3.4 μm) HPLC Columns

Name: BIOshell<SUP>&#8482;</SUP> A400 Protein C18 (3.4 μm) HPLC Columns
Brand: SUPELCO

L × I.D. 5 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:

41115700

NACRES:

SB.52

L × i.d.:

5 cm × 2.1 mm

Particle size:

3.4 μm

Matrix active group:

C18 (octadecyl) phase

Pore size:

3.4 μm particle size, 400 Å

Matrix:

spherical silica particle platform, superficially porous particle

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Properties

Product Name

BIOshell^™ A400 Protein C18 Column, L × I.D. 5 cm × 2.1 mm, 3.4 μm particle size

material

stainless steel hardware (column)

Quality Level

100

agency

suitable for USP L1

description

Shell Thickness (0.2 μm), Solid Core (3.0 μm)

product line

BIOshell^™

feature

endcapped

analyte chemical class(es)

proteins

manufacturer/tradename

BIOshell^™

packaging

1 ea of

extent of labeling

1% carbon loading

parameter

600 bar max. pressure, 90 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

5 cm × 2.1 mm

surface area

~15 m²/g

matrix

spherical silica particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

3.4 μm

pore size

3.4 μm particle size, 400 Å

operating pH

1-8

separation technique

reversed phase

Description

General description

BIOshell^™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core^® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 1 to 9.

Application

UHPLC Analysis of Proteins on BIOshell^™ A160 Peptide C18 2.7 μm; BIOshell^™ A400 Protein C18, 3.4 μm; and BIOshell^™ IgG 1000 Å C18, 2.7 μm

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Global Trade Item Number

SKU	GTIN
67459-U	04061833364970