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TSKgel® ODS-100V HPLC Column

phase C18 (octadecyl), L × I.D. 15 cm × 4.6 mm, 5 μm particle size

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Synonym(s):
TSKgel® Reversed Phase HPLC Column
eCl@ss:
32110501

material

stainless steel hardware

Agency

suitable for USP L1

product line

TSKgel®

manufacturer/tradename

Tosoh ODS-100V, 21455

extent of labeling

15% Carbon, endcapped with bifunctional dialkylsilane

parameter

0-100% organic solvent
1.0 mL/min standard flow rate
10-50 °C temperature
180 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

450 m2/g

matrix

silica particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

100 Å

operating pH

2.0-7.5

separation technique

reversed phase

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General description

TSKgel® ODS-100V and TSKgel ODS-100Z columns incorporate the best-in-class surface properties to limit secondary interactions of basic, acidic and chelating compounds. Take advantage of the benefits of high column efficiency and symmetrical peak shapes to reduce your analysis and method development time! The ultra high purity Type B base silica contains negligible amounts of metal ion impurities. Combined with monomeric bonding chemistry, this silica makes the best general purpose Reversed Phase columns suitable for the most demanding separations in quality control as well as in research and development. TSKgel ODS-100V columns have a lower carbon content than ODS-100Z columns, making the bonded phase surface 100% water wettable and the preferred choice to retain polar metabolites.

Application

TSKgel® ODS-100V HPLC Column may be used for the separation of:
  • Docetaxel in human plasma during its determination by high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) combined with electrospray ionization (ESI) and multiple reaction monitoring (MRM) detection.
  • Constituents of Chinese medicine Niu Huang Jie Du Pill during their analysis by HPLC combined with diode array detection (DAD).
  • Organic and inorganic selenium in diet supplements during the determination using HPLC-ESI-MS/MS with MRM detection.
  • Estrogenic compounds 8-prenylnaringenin, zearalenone, α- and β-zearalenol in beer during their identification using LC-ESI-MS with single ion monitoring (SIM) detection.
  • Lincomycin, spectinomycin and its impurities in pharmaceutical products during their analysis by HPLC combined with charged aerosol detection (CAD) as well as HPLC coupled to time-of flight mass spectrometer (TOFMS) with ESI.

Physical form

Shipped in acetonitrile.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Regulatory Information

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A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study.
Yamaguchi H, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 893, 157-161 (2012)
Qualitative and quantitative analysis of traditional Chinese medicine Niu Huang Jie Du Pill using ultra performance liquid chromatography coupled with tunable UV detector and rapid resolution liquid chromatography coupled with time-of-flight tandem mass spectrometry
Liang X, et al.
Journal of Pharmaceutical and Biomedical Analysis, 51(3), 565-571 (2010)
Simultaneous quantitation and identification of organic and inorganic selenium in diet supplements by liquid chromatography with tandem mass spectrometry.
Zembrzuska J, et al.
Food Chemistry, 142(3), 178-187 (2014)
Development of high performance liquid chromatography methods with charged aerosol detection for the determination of lincomycin, spectinomycin and its impurities in pharmaceutical products
Stypulkowska K, et al.
Journal of Pharmaceutical and Biomedical Analysis, 112(1), 8-14 (2015)
Direct determination of the estrogenic compounds 8-prenylnaringenin, zearalenone, ?- and ?-zearalenol in beer by liquid chromatography-mass spectrometry.
Maragou NC, et al.
Journal of Chromatography A, 1202(1), 47-57 (2008)

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