Affinity Chromatography Resins
Affinity chromatography is a powerful purification technology that separates biomolecules through highly selective interactions. This exceptional selectivity streamlines impurity removal and achieves high purity in a single step. Our portfolio of affinity resins addresses diverse process needs and provides the purification tools you need from platform manufacturing to next-generation applications.
Amsphere® Protein A Resins Are Now Part of Our Portfolio
Amsphere® resins are well-established Protein A resins for efficient purification of antibodies and their derivatives. This expanded resin portfolio offers more options for you to optimize process performance, enhance productivity, and improve economics.
Protein A Affinity Resins for Antibody Capture
Amsphere® A+: Next-generation Protein A affinity resin engineered for superior process efficiency
- Outstanding dynamic binding capacity optimized for high-titer and intensified processes
- Excellent durability and caustic stability for enhanced bioburden control
- Superior pressure-flow properties and impurity removal ability
Amsphere® A3: Well-established high-capacity Protein A resin with an extensive track record in clinical and commercial processes.
- High dynamic binding capacity optimized for high-titer processes and process intensification
- Excellent productivity and cost-of-goods optimization at manufacturing scale
- Platform affinity resin for standard mAbs and antibody derivatives including Bi-specifics, Fc-fusion, fragments, VHH single-domain antibodies
Eshmuno® A: Proven and reliable Protein A resin designed for robust and scalable monoclonal antibody purification.
- High dynamic binding capacity and reliable performance across a wide operating window and process conditions
- Caustic stability for enhanced bioburden control
- Excellent pressure-flow properties and packing convenience at large scale
Affinity Resins for AAV Capture Purification
NEW Eshmuno® AAV9 is an affinity resin designed for capture purification of adeno-associated virus serotype 9 (AAV9), a commonly used vector for viral gene therapy production. This novel resin combines high flow rates and excellent binding capacity with high recovery and caustic stability and offers a path to boost efficiency of AAV9 production while reducing manufacturing costs.
Affinity Resins for Plasma Purification
Eshmuno® P anti-A and anti-B resins effectively remove anti-A and anti-B isoagglutinin antibodies in plasma-derived Immunoglobulin (Ig) processes, which has been associated with increased patient risk for hemolysis.
Affinity Resins for Protein Purification
Fractogel® Chelate resin offers efficient and cost-effective separation of proteins and peptides, especially proteins with exposed histidine, cysteine, and tryptophan residues, including histidine-tagged proteins.
Highlights and Support Available
- High-performance resins for purification of antibodies and antibody derivatives
- Extensive technical and application expertise to support process development and scale-up
- Global manufacturing and supply chain network ensuring supply resilience and operational continuity
- Regulatory excellence with the EMPROVE® Program providing full transparency and comprehensive documentation to support qualification, risk assessment, and process optimization to simplify your compliance needs.
Related Webinars
Strategies to address Immunoglobulin purification challenges by using Ion Exchange and Affinity chromatography media to enhance both purity & yield.
Learn productivity & COGs calculations of Protein A affinity chromatography resin at production scale, including best practice strategies to lower costs.
This webinar will cover our study design for extractables profiles of chromatography resins within our Emprove® Program.
Related Documents
- Datasheet: Eshmuno® AAV9 Chromatography Resin
Explore how our new affinity resin can advance your purification process.
- Data Sheet: Amsphere® A+ Protein A Affinity Resin
Learn more about our next-generation resin, engineered for superior process efficiency.
- Data Sheet: Amsphere® A3 Protein A Resin
Proven Protein A solution for reliable purification of antibodies and derivatives.
- User Guide: Biochromatography Portfolio
Bulk resin, membranes and prepacked columns.
- User Guide: Chromatography Purification Product Selection
Chromatography product selection guide.
- Data Sheet: Eshmuno® A Resin
Eshmuno® A media is a rigid, high-capacity, acid and alkaline resistant Protein A affinity chromatography media for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies.
- Data Sheet: Eshmuno® P anti-A and anti-B Resins
High performance, acid and alkaline resistant affinity chromatography resins designed for the removal of anti-A and anti-B antibodies from plasma-derived immunoglobulin (Ig).
- Data Sheet: Fractogel®Chromatography Resin
Fractogel® resins consist of synthetic methacrylate based polymeric beads providing excellent pressure stability resulting in high flow rates. Depending on your purification application, we can offer resin sized M-type beads with a particle size of 40–90 μm and small S-type beads with a particle size in the range of 20–40 μm.
- Application Note: Increase your Eshmuno® A resin lifetime with Ethylene Glycol Emprove® Evolve
Sodium hydroxide (NaOH) is a standard cleaning agent for protein A chromatography resins. Typically, a concentration of 0.1 mol/L NaOH is used. The inclusion of 20% ethylene glycol in a 0.1 mol/L NaOH cleaning solution increases the lifetime of Eshmuno® A columns significantly.
- Application Note: Detection and Quantification of Leached Protein A from Eshmuno® A Resin
Using Cygnus F610 Protein A Mix-N-Go™ ELISA Kit.
