(800) 437-7500 • Fax: +1 (951) 676-9209 • Europe +44 (0) 23 8026 2233
Australia +61 3 9839 2000
www.millipore.com
04-22-08/SCM060/VC/AN
7. Resuspend the cells in a total volume of 10 mL of
(800) 437-7500 • Fax: +1 (951) 676-9209 • Europe +44 (0) 23 8026 2233
Australia +61 3 9839 2000
www.millipore.com
06-20-08/SCM041/VC/AN
7. Resuspend the cells in a total volume of 10 mL of
Cell. Biol., 36(22):
2855-2866 (2016).
6. Cheng, F. et al., Mol. Immunol., 60(1):
44-53 (2014).
7. Singh, G. et al., Methods, 65(3):
320-332 (2014).
8. Meller, N. et al., J. Biol.
Escherichia coli
8739
10-100
44-48 h at 30-35
°C
70-200 %; good growth;
reddish, medium-sized
colonies
Pseudomonas
aeruginosa
9027 10-100 44-48 h at 30-35
°C
-8 -7 -6 -5 -4
-10
0
10
20
30
40
50
60
70
80
90
100
110
120
[Carbachol] Log M
∆∆ ∆∆
re
la
ti
ve
l
u
m
in
es
ce
nc
e
u
n
it
s
(%
m
ax
im
u
m
)
-10 -9 -8
(800) 437-7500 • Fax: +1 (951) 676-9209 • Europe +44 (0) 23 8026 2233
Australia +61 3 9839 2000
www.millipore.com
04-22-08/SCM040/VC/AN
7. Resuspend the cells in a total volume of 10 mL of
Clostridium
perfringens
13124 100-1000
44-48 h at 35-37 °C
(anaerobic)
good growth; grey-whitish,
large, arched colonies
Clostridium
sordellii
9714 100-1000
44-48 h at 35-37 °C
(anaerobic
column 5 to column 7 and
100 µL from column 6 to column 8. After mixing (10x), transfer 100 µL from
the wells in column 5 to the wells in column 7 and 100 µL of wells in
column 6 to the wells
Clostridium
perfringens
13124 100-1000
44-48 h at 35-37 °C
(anaerobic)
good growth; grey-whitish,
large, arched colonies
Clostridium
sordellii
9714 100-1000
44-48 h at 35-37 °C
(anaerobic
was extremely difficult. Incu-
bation of LTTC at 44 ± 0Æ5°C slightly improved this lack of
selectivity, but in 52% (80/155) of the samples, 50–100% of
the membrane filter surface remained covered
Result
Escherichia coli 8739 10-100 44-48 h at 30-35 °C
70-200 %; good growth;
reddish, medium-sized
colonies
Pseudomonas
aeruginosa
9027 10-100 44-48 h at 30-35 °C
70-200 %; good growth