Figure 2.
Modeled API/PVA4-88 phase diagrams of (A) IND/PVA4-88, (B) NAP/PVA4-88 and (C) ITR/PVA4-88 containing the PC-SAFT modeled crystalline solubility in amorphous PVA4-88 (magenta line) and the
Kwei
of the various BPC components
(a 100% extraction) and then use these concentrations to
demonstrate biological compatibility per modified USP
and 88> methodology.
The bag surface area-to-volume
Complete volume to 88 µl with lysis buffer.
3. Add 88 µl of lysis buffer to the Blank control tube.
4. Add 2 µl of 25 µg/ml MGMT to the Positive control
tube and complete volume to 88
determined by HPLC or SDS-PAGE.
Endotoxin level: ≤10 EU/ml (LAL).
Specific Activity
≤100 MU/mg
(hIL-6, NIBSC standard 88/514), at least the same specific activity (EC50 ) compared to the indicated standard
volume of 100 ml.
Remove insoluble material by filtration.
Solution is stable for at least 1 month when stored in a dark bottle in
refrigerator at 0-5°C.
Sigma Tech. Bulletin No. MWS.877L (7-88,
3
volume of 100 ml.
Remove insoluble material by filtration.
Solution is stable for at least 1 month when stored in a dark bottle in
refrigerator at 0-5°C.
Sigma Tech. Bulletin No. MWS.877L (7-88,
3
glycerol.
Molecular mass: ∼65 kDa
Purity: 80–95% (SDS-PAGE, see Figure 1)
Specific Activity: 64–88 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
Toxicity1 Component materials were tested and meet the criteria of USP 88> Reactivity Test Class VI Plastics, and
are non-toxic per the USP 88> Reactivity Safety Test.
Integrity Test Specification1 — Air/