TSA with incubation at 37°C for 24 hours for the negative control, E.coli and E. faecalis cultures in accordance
with ISO 11290-2 AMD. The criteria were more than 103 cfu/mL of L. monocytogenes in the
Application Note
2
Figure 1. High levels of SEAP expression is evident 24 h post-
transfection. Levels of SEAP secreted into the growth medium
reached 1050 ng/mL 24 h after transfection; an
Expected Results
Escherichia coli 25922 103-105 24 h at 35°C
Recovery rate % (with
and without blood) >70
Staphylococcus
aureus
25923 103-105 24 h at 35°C
Recovery rate % (with
and
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Cultural characteristics with ESBL ChromoSelect Selective Supplement after 24 hours at 35-37°C.
References:
The first enrichment is performed in 1/2 Fraser broth (dilution 1:10, e.g. 25g sample in 225ml 1/2 Fraser broth).
The 1/2 Fraser broth is incubated at 30°C for 24 ±3 hours. Using an inoculating loop, a
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Page 2 of 2
Cultural characteristics with Strep B ChromoSelect Selective Supplement after 18-24 hours at 35-37°C.
References:
reagents.2 To further minimize the risk of contaminant DNA during
PCR, we include 103 MTP Taq Buffer (Catalog Number M9943)
with each tube of MTP Taq DNA Polymerase. Each lot of MTP Taq
and 103 MTP Taq