0.01-100 µg/ml, recombinant mouse IGF-II at 30 ng/ml,
and cells at 5 x 104 cells/ml, is incubated at 37 °C for
72 hours in a humidified CO2 incubator. The mixture is
pulsed
average of 104% recovery was calculated (figure 1). With
m-Endo, the average recovery was 113% (figure 2), while
with m-Green Yeast and Mold broth, recovery was 95%
(figure 3).
The average
concentrations of 0.01-100 µg/ml, recombinant
mouse IGF-I at 15 ng/ml, and cells at 5 x 104 cells/ml, is
incubated at 37 °C for 72 hours in a humidified CO2
incubator. The mixture is pulsed with 3H-thymidine
/ml), yielding a
clear, colorless solution. It is also soluble in alcohol
(82 mg/ml), glycerol (104 mg/ml), and in isopropanol
(21 mg/ml).1
Storage/Stability
The pH of a 1% aqueous solution of this
concentrations of 0.1-100 µg/mL,
recombinant mouse VEGF at 5 ng/mL, and cells at 5 x
104 cells/mL, is incubated at 37 °C for 72 hours in a
humidified CO2 incubator. The mixture is pulsed with
3H-thymidine
performed 24 to 72 hours following
tranfection, depending on the nature of the cell
type and nature of the reporter gene.
Note: The same protocol can be used to produce
stably transfected cells, 48-72 hours post
Fleming, H. Levison, Pseudomonas
cepacia in cystic fibrosis Infection: An Emerging problem. J. Pediatr. 104:206-210. (1984)
7. J.J. MILLER, A guide to Specimen Management in Clinical Microbiology. ASM Press
application, cells should be added to the gel
prior to plating at a recommended density of
3-4 104 cells per mL.
To dissociate cells from the gel, use protease
(dispase) in PBS without calcium
human Interleukin-1α at 50 pg/mL, Concanavalin A at
1.25 µg/mL and cells at 5 x 104 cells/mL, was incubated
at 37 °C for 72 hours in a humidified CO2 incubator.
3H-thymidine was added during the final
recombinant murine IL-1α at
50 pg/mL, Concanavalin A at 1.25 mg/mL, and cells at
5 x 104 cells/mL, was incubated at 37 °C for 72 hours in
a humidified CO2 incubator. 3H-thymidine was added
during the final
human
Interleukin-1α at 50 pg/mL, Concanavalin A at 1.25
mg/mL, and cells at 5 x 104 cells/mL, was incubated at
37 °C for 72 hours in a humidified CO2 incubator.
3H-thymidine was added during the final
12 hours.
Product Profile
The recommended working dilutions are 1:105 to 1:106
for ELISA; 1:104 to 1:105 for immunoblotting; 1:10 to
1:100 for immunoprecipitation, and 1:100 to 1:1000 for
immunohistochemistry
techniques for future analysis.)
5 x 104 cells per well of a 6-well plate; 6 wells total with 2 ml of MEF
Medium per well.
2 x 104 cells per well in a 4-well plate; 3 plates total
.25 3–4
1 well of a 12 well plate 3.80 300 0.1 2
1 well of a 24 well plate 2.00 200 0.05 1
1 well of a 48 well plate 0.75 100 0.02 0.5
1 well of a 96 well plate 0.32 50
.25 3–4
1 well of a 12 well plate 3.80 300 0.1 2
1 well of a 24 well plate 2.00 200 0.05 1
1 well of a 48 well plate 0.75 100 0.02 0.5
1 well of a 96 well plate 0.32 50