x 104 0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish
x 104 0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish
x 104 0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish
x 104 0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish
x 104 0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish
see “Determination procedure”, section 2
Drying cabinet (105 °C)
Desiccator with drying agent
Glass beakers (400 ml, 250 ml, 600 ml)
Waterbath with thermostat, 60 °C and 100 °C, equipped with magnetic
see “Determination procedure”, section 2
Drying cabinet (105 °C)
Desiccator with drying agent
Glass beakers (400 ml, 250 ml, 600 ml)
Waterbath with thermostat, 60 °C and 100 °C, equipped with magnetic
siehe „Durchführung des Tests“, Abschnitt 2
Trockenschrank (105 °C)
Exsikkator mit Trockenmittel
Bechergläser (400 ml, 250 ml, 600 ml)
Wasserbad mit Thermostat, 60 °C und 100 °C, ausgerüstet mit Magnetrührer
except seed 250 mL shakers at
6 x 105 cells/mL in 60 mL at 60 rpm.
14. After the cultures have been established in EX-
CELL® 302, passage the cells for an additional
2 passages in 250 mL
seed 250 mL shaker flasks
at 6 x 105 cells/mL in 60 mL at 60 rpm.
14. After the cultures have been established in
EX-CELL® 302, passage the cells for an additional
2 passages in 250 mL
seed 250 mL shaker flasks at
6 x 105 cells/mL in 60 mL at 60 rpm.
14. After the cultures have been established in
EX-CELLTM 302, passage the cells for an additional
2 passages in 250 mL shaker flasks