Rev 04/22 5 of 12
Table 2 .
Average
Concentration
(ng/µl)
sheared
Average
Concentration
(ng/µl)
not sheared
4 x 106 cells 39.77 25.15
2 x 106 cells 18.73 15.03
Histone Surface
Accessibilities". University of Rochester, Ph.D.
dissertation, pp. 37, 69, 106 (2020).
22. O’Kane, Callum Jude, "Probing the role of histone
acetylation in the evolution of pathogenicity
Texas
Southwestern Medical Center at Dallas, Ph.D.
dissertation, p. 106 (2018).
16. Mucenski, M.L. et al., Sci. Rep., 9(1), 4557
(2019).
17. Bonneau, P.R. et al., Bioorg. Chem., 21(4),
431
spike membrane glycoprotein.
The nucleic acid sequence encoding this peptide
(amino acids 98-106 of influenza HA) has been
incorporated into various expression plasmids
adjacent to the cloning site
100 µl of diluent. Incu-
bate at room temperature (18 - 22 °C) for
30 minutes. Protect from light at this and all
subsequent steps.
9. a. If whole blood is used, use lysing solution after
add 100 µl of
diluent. Incubate at room temperature (18 -
22°C) for 30 minutes. Protect from light at this
and all subsequent steps.
9. a. If whole blood is used, use lysing solution
after incubation
the infectivity of the human
virus.6 The HA tag is a short sequence derived from
amino acids 98-106 of the HA molecule. Many
recombinant proteins have been engineered to
express the HA tag, which does
100 µl of diluent. Incu-
bate at room temperature (18 - 22 °C) for 30 min-
utes. Protect from light at this and all subsequent
steps.
9. a. If whole blood is used, use lysing solution after
incubation
100 µl or 1 x 106 cells per
tube.
2. Add 5 µl of monoclonal antibody to tube(s) contain-
ing cells to be stained. Vortex tube gently. Incu-
bate the cells at room temperature (18–22 °C) for
1 x 106 cells per
tube.
2. Add 10 µl of biotinylated monoclonal antibody to
tube(s) containing cells to be stained. Vortex tube
gently to mix. Incubate the cells at room tempera-
ture (18 – 22 ° C)
100 µl or 1 x 106 cells
per tube.
2. Add 5 µl of monoclonal antibody to tube(s) contain-
ing cells to be stained. Vortex tube gently. Incu-
bate the cells at room temperature (18 – 22 °C) for
100 µl or 1 x 106 cells
per tube.
2. Add 5 µl of monoclonal antibody to tube(s)
containing cells to be stained. Vortex tube gently to
mix. Incubate the cells at room temperature (18 -
22 °C) for 30 minutes
the protein(s)
of interest.
Procedure
This procedure is suitable for extraction of
106 to 107 cells. If larger numbers of cells are to be
used, or multiple extractions are run
µl or 1 x 106 cells per
tube.
2. Add 5 µl of monoclonal antibody to tube(s)
containing cells to be stained. Vortex tube gently.
Incubate the cells at room temperature
(18
add 100 µl or 1 x 106 cells per
tube.
2. Add 5 µl of antibody to tube(s) containing cells to be
stained. Vortex tube gently. Incubate the cells at
room temperature (18 – 22 °C)
100 µl or 1 x 106 cells
per tube.
2. Add 5 µl of monoclonal antibody to
tube(s)
containing cells to be stained. Vortex
tube gently. Incubate the cells at room
temperature (18 – 22 °C)
titration assay.
When assayed by flow cytometric analysis, using 1 µg
of the antibody to stain 1 X 106 cells, a fluorescence
intensity is observed similar to that obtained with
saturating monoclonal
1 x 106 cells per
tube.
2. Add 10 µl of biotinylated monoclonal antibody to
tube(s) containing cells to be stained. Vortex tube
gently to mix. Incubate the cells at room tempera-
ture (18 - 22°C) for
100 µl or 1 x 106 cells per
tube.
2. Add 5 µl of monoclonal antibody to tube(s) con-
taining cells to be stained. Vortex tube gently. Incu-
bate the cells at room temperature (18 – 22 °C)
100 µl or 1 x 106 cells per
tube.
2. Add 5 µl of monoclonal antibody to tube(s)
containing cells to be stained. Vortex tube gently.
Incubate the cells at room temperature (18 – 22 °C)
for
infectivity of the virus.6
The HA-Tag consists of a short sequence that
corresponds to amino acids 98-106 of HA. Many
recombinant proteins have been engineered to express
the HA-Tag, which does not appear