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Showing 1-30 of 1035 results for "106-30-9" within Technical Documents
Cell Culture Media 4CHO Sample Kit
trial samples 1 0.5 x 106 1.5 x 106 3 x 106 10 5.0 x 106 15 x 106 30 x 106 15 7.5 x 106 22.5 x 106 45 x 106 30 15 x 106
Combat Microbes with ProClin Biocidal Preservatives
Broth Agar 3.38 x 106 4.2 x 106 3.30 x 106 6.5 x 106 a Potato Dextrose Agar 1.93 x 106 2.1 x 106 3.56 x 106 1.31 x 107 a Sodium Azide Tryptic Soy Broth Agar
Bulletin - CHROP
new 1.5 mL tube, or to several tubes in conveniently-sized aliquots for storage. Chromatin from 10 9. × 106 cells (˜100 µL) will be used per ChIP for a rare transcription factor target. See Table 1 for recommended
The Effective Use of Protein Kinase Inhibitors
2 5 . 5 8 3 2 84 99 99 9 64 59 20 90 93 80 94 103 96 52 55 89 82 89 99 94 102 95 86 101 105 106 34 37 57 87 81
Product Information Sheet - BK101
∼150,000) nmoles/ml = mg protein/ml × 106 mw of IgG (106 is conversion from moles/L to nmoles/ml) nmoles/ml = 4.5 mg/ml × 106 150,000 nmoles/ml = 30 3. To determine the number of nmoles
Product Information Sheet - 11663925910
hyorhinis Vero 106 1.68 +++ ++ +++ +++ Vero 103 1.85 ++ + +++ +++ NSO 106 1.83 +++ ++ +++ ++ NSO 103 1.78 + + ++ + M. orale Vero 106 0.49 +++ ++++ +++ +++ Vero 103 0.24 ++ +
The Effective Use of Protein Kinase Inhibitors
www.sigma-aldrich.com/cellsignaling 84 99 99 9 64 59 20 90 93 80 94 103 96 52 55 89 82 89 99 94 102 95 86 101 105 106 34 37 57 87 81 26
Upstream process intensification using frozen high cell density intermediates
different cell densities from 10 x 106 VC/mL (control) up to 100 x 106 VC/mL. Vials were thawed in a water bath at room temperature as previously described, used for inoculation of 30 mL bioreactor tubes, and growth
Process Guidance - Cellvento BHK-200
the culture is stable with a viability of > 95% and a cell density above 1.5×106 viable cells/mL - Prepare a MCB (min. 30 tubes in Cellvento® BHK-200 medium + 10% DMSO, 1×107 viable cells/mL)
Data Sheet - 102376
Passage when VCD is above 1.5 × 106 cells/mL • When the culture is stable with a viability of > 95% and a cell density above 1.5 × 106 cells/mL. • Prepare a MCB (min. 30 tubes in
Validation and Qualification for Durapore® Sterilizing-Grade (0.22 µm) Membrane VMF4
116 45 13.4 7.64 101 39 10.8 3.95 115 26 13.4 7.66 106 19 10.2 4.01 114 58 14.1 7.75 91 51 11.0 4.11 90 27 13.6 7.76 109 50 11.0 4.13 89 9 13.1 7.99
Data Sheet - G0791
Gene Therapy Medium-2. Add 9 ml of fresh Gene Therapy Medium-2. 7. Transfer the cell suspension to a T-75 flask containing fresh Gene Therapy Medium-2 at a final volume of 30 ml. Adaptation to
Product Information Sheet - PISTAIN
at room temperature. 9. Aspirate supernatant and resuspend pellet in 500 l of citrate buffer solution. 10. Using citrate buffer solution, adjust the cell concentration to 2.5 x 106 cells/ml. IV. Paraffin-embedded
Product Information Sheet - Z406260
Z40,627-9 Z40,629-5 1000mL Z40,628-7 Z40,630-9 Transfer needles 12 gauge SS, double-ended with one deflecting tip and one flat-cut end. For fabrication of transfer lines with CHEM-FLEX™ 106 tubing.
Monoclonal Anti-CD8-Biotin antibody produced in mouse (B0406)
µl of diluent. Incu- bate at room temperature (18 - 22 °C) for 30 min- utes. Protect from light at this and all subsequent steps. 9. a. If whole blood is used, use lysing solution after incubation
EmbryoMax® PMEF, Strain DR4, Irradiated, passage 3
75 cm2 flask 12 mL 75 cm2 3.75 x 106 25 cm2 flask 6 mL 25 cm2 1.25 x 106 100 mm plate 10 mL 56 cm2 2.8 x 106 60 mm
EmbryoMax® PMEF, Strain DR4, Mytomycin C treated, passage 3
75 cm2 flask 12 mL 75 cm2 3.75 x 106 25 cm2 flask 6 mL 25 cm2 1.25 x 106 100 mm plate 10 mL 56 cm2 2.8 x 106 60 mm
EmbryoMax® PMEF, Strain DR4, Irradiated, passage 3
75 cm2 flask 12 mL 75 cm2 3.75 x 106 25 cm2 flask 6 mL 25 cm2 1.25 x 106 100 mm plate 10 mL 56 cm2 2.8 x 106 60 mm
Microsoft Word - VP001 tech bulletin final
allow cells to reach viable cell density (VCD) greater than 8  106 viable cells/mL for optimal performance. Discard cells after 30 passages. 3. Subculture cells for at least three passages prior
Product Information Sheet - SHM02
1.0 6 well 2-5 x 105 1-2 x 106 30 200 2.0 60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0 90-100 mm dish 15-30 x 10 5 5-10 x 106 150 800 8.0 T-25 flask 5-10 x
Product Information Sheet - SHM05
1.0 6 well 2-5 x 105 1-2 x 106 30 200 2.0 60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0 90-100 mm dish 15-30 x 10 5 5-10 x 106 150 800 8.0 T-25 flask 5-10 x
Product Information Sheet - SHM03
1.0 6 well 2-5 x 105 1-2 x 106 30 200 2.0 60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0 90-100 mm dish 15-30 x 10 5 5-10 x 106 150 800 8.0 T-25 flask 5-10 x
Monoclonal Anti-CD15-Biotin antibody produced in mouse (B0906)
. Product Profile When assayed by flow cytometric analysis, using 10 µl of the antibody per 1 X 106 cells and ExtrAvidin -FITC Conjugate (Product No. E 2761), a fluorescence intensity is observed similar
Product Information Sheet - SHM01
1.0 6 well 2-5 x 105 1-2 x 106 30 200 2.0 60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0 90-100 mm dish 15-30 x 10 5 5-10 x 106 150 800 8.0 T-25 flask 5-10 x
Product Information Sheet - SHM04
1.0 6 well 2-5 x 105 1-2 x 106 30 200 2.0 60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0 90-100 mm dish 15-30 x 10 5 5-10 x 106 150 800 8.0 T-25 flask 5-10 x
Monoclonal Anti-B23 antibody produced in mouse (B0556)
µl of diluent. Incu- bate at room temperature (18 - 22 °C) for 30 minutes. Protect from light at this and all subsequent steps. 9. a. If whole blood is used, use lysing solution after incubation
MONOCLONAL ANTI-HUMAN CD16 CLONE BL-LGL/1 Purified Mouse Immunoglobulin
or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) contain- ing cells to be stained. Vortex tube gently. Incu- bate the cells at room temperature (18–22 °C) for
Data Sheet - C6555
stained, i.e. 1 µg of monoclonal antibody per 1 x 106 cells in a final volume of 100 µl. Tap tube gently to mix. Incubate the cells on ice for 30 minutes. Proper controls to
FlowCellect™ Mouse TH1/TH2 Intracellular Cytokine Kit
for 15 minutes in the dark. 9. Spin down cells at 2500 rpm (600Xg) for 3 minutes and discard buffer. 10. Resuspend the cells in 1mL of 1X Assay Buffer/5X 106 cells by gently pipetting up
FlowCellect™ Mouse TH1 Intracellular Cytokine Kit
for 15 minutes in the dark. 9. Spin down cells at 2500 rpm (600Xg) for 3 minutes and discard buffer. 10. Resuspend the cells in 1mL of 1X Assay Buffer/5X 106 cells by gently pipetting up
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