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Cell Culture Media 4CHO Sample Kit
trial samples
1 0.5 x 106 1.5 x 106 3 x 106
10 5.0 x 106 15 x 106 30 x 106
15 7.5 x 106 22.5 x 106 45 x 106
30 15 x 106
Combat Microbes with ProClin Biocidal Preservatives
Broth Agar 3.38 x 106 4.2 x 106 3.30 x 106 6.5 x 106 a
Potato Dextrose Agar 1.93 x 106 2.1 x 106 3.56 x 106 1.31 x 107 a
Sodium Azide
Tryptic Soy Broth Agar
User Guide: Sf-RVN® Platform
at 0.5 x 106 cells per mL in 30 mL
of EX-CELL® CD Insect Cell Medium in a 125 mL flask.
2. Prepare the reaction tubes for each flask for the
recommended lowest (3 μL) and highest (30 μL)
amount
Bulletin - CHROP
new 1.5 mL tube, or to several tubes in conveniently-sized aliquots for storage. Chromatin from 10 9. × 106
cells (˜100 µL) will be used per ChIP for a rare transcription factor target. See Table 1 for recommended
Critical evaluation on the use of Dmb-dipeptides in the synthesis of neurotoxic prion peptide
resin (0.1 mmol, 0.71 mmol/g)
Instrument ABi 433A
Coupling
Fmoc-Aaa-OH: HBTU:HOBt:DIPEA (10:9:9:20), 30 mins
Fmoc-Aaa-(Dmb)Gly-OH (3:3:3:6), 60 min
Deblock 20% Piperidine (3 or 4 times 3 min)
Critical evaluation on the use of Dmb-dipeptides in the synthesis of neurotoxic prion peptide
resin (0.1 mmol, 0.71 mmol/g)
Instrument ABi 433A
Coupling
Fmoc-Aaa-OH: HBTU:HOBt:DIPEA (10:9:9:20), 30 mins
Fmoc-Aaa-(Dmb)Gly-OH (3:3:3:6), 60 min
Deblock 20% Piperidine (3 or 4 times 3 min)
The Effective Use of Protein Kinase Inhibitors
2
5
.
5
8
3
2
84 99 99 9 64 59 20 90 93 80 94 103 96 52 55 89 82 89 99 94 102 95
86 101 105 106 34 37 57 87 81
Product Information Sheet - 11663925910
Store 2 weeks at +2 to +8°C, or
2 days at +20 to +25°C, or 8 hours at
+30°C.
9 Conjugte dilution
buffer (Bottle 9)
Ready-to-use solution. After opening, store at +2 to +8°C.
10 TMB
Product Information Sheet - BK101
∼150,000)
nmoles/ml = mg protein/ml × 106
mw of IgG
(106 is conversion from moles/L to nmoles/ml)
nmoles/ml = 4.5 mg/ml × 106
150,000
nmoles/ml = 30
3. To determine the number of nmoles
The Effective Use of Protein Kinase Inhibitors
www.sigma-aldrich.com/cellsignaling
84 99 99 9 64 59 20 90 93 80 94 103 96 52 55 89 82 89 99 94 102 95
86 101 105 106 34 37 57 87 81 26
Process Guidance Cellvento™ BHK-200 Medium
the culture is stable with a viability of > 95% and a cell density above 1.5×106 viable cells/mL
– Prepare a MCB (min. 30 tubes in Cellvento™ BHK-200 medium + 10% DMSO, 1×107 viable cells/mL)
– Prepare
Mobius®CellReady 50 L and 200 L Single-use Bioreactor Systems Performance Guide
k L
a
[h
r-
1 ]
100 L 200 L40 L
Impeller rpm
0
106 rpm [5 W/m3] 133 rpm [10 W/m3] 192 rpm [25 W/m3]
5
10
15
20
25
30
35
40
45
50
Ti
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[s
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Upstream process intensification using frozen high cell density intermediates
different
cell densities from 10 x 106 VC/mL (control) up to 100 x 106
VC/mL. Vials were thawed in a water bath at room temperature as
previously described, used for inoculation of 30 mL bioreactor
tubes, and growth
Insect Directﾙ Protein Expression & Purification System
+ 4 ml 5 ml 50 µl 2.5 ml 30 mm × 20 cm 2 × 15 ml 7.5 ml
200 ml 180 ml at
1.1 × 106/ml
400 µg + 10 ml 2 ml + 8
Process Guidance - Cellvento BHK-200
the culture is stable with a viability of > 95% and a cell density
above 1.5×106 viable cells/mL
- Prepare a MCB (min. 30 tubes in Cellvento® BHK-200 medium +
10% DMSO, 1×107 viable cells/mL)
Product Information Cellvento™ BHK-200 Medium
Passage when VCD is above 1.5 × 106 cells/mL
• When the culture is stable with a viability of > 95% and a cell density
above 1.5 × 106 cells/mL
• Prepare a MCB (min. 30 tubes in Cellvento™ BHK-200
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
51 10730 97 14 45 24 34 30 135 25
GDF8 std
4
19 11 14 15 100 39 26 425 14 43 24 30 27 115 25
19 12 17 15 106 38 25 421 13
Data Sheet - 102376
Passage when VCD is
above 1.5 × 106 cells/mL
• When the culture is stable with a viability of > 95%
and a cell density above 1.5 × 106 cells/mL.
• Prepare a MCB (min. 30 tubes in
Validation and Qualification for Durapore® Sterilizing-Grade (0.22 µm) Membrane VMF4
116 45 13.4 7.64 101
39 10.8 3.95 115 26 13.4 7.66 106
19 10.2 4.01 114 58 14.1 7.75 91
51 11.0 4.11 90 27 13.6 7.76 109
50 11.0 4.13 89 9 13.1 7.99
Data Sheet - G0791
Gene Therapy Medium-2. Add 9 ml of fresh
Gene Therapy Medium-2.
7. Transfer the cell suspension to a T-75 flask
containing fresh Gene Therapy Medium-2 at a final
volume of 30 ml.
Adaptation to
Product Information Sheet: Cellvento® BHK-200 cell culture medium
Passage when VCD is
above 1.5 × 106 cells/mL
• When the culture is stable with a viability of > 95%
and a cell density above 1.5 × 106 cells/mL.
• Prepare a MCB (min. 30 tubes in
Product Information Sheet - PISTAIN
at room temperature.
9. Aspirate supernatant and resuspend pellet in 500 l of citrate buffer solution.
10. Using citrate buffer solution, adjust the cell concentration to 2.5 x 106 cells/ml.
IV. Paraffin-embedded
Product Information Sheet - Z406260
Z40,627-9 Z40,629-5
1000mL Z40,628-7 Z40,630-9
Transfer needles
12 gauge SS, double-ended with one deflecting tip and one flat-cut end. For
fabrication of transfer lines with CHEM-FLEX™ 106
tubing.
EmbryoMax® PMEF, Strain DR4, Irradiated, passage 3
75 cm2 flask 12 mL 75 cm2 3.75 x 106
25 cm2 flask 6 mL 25 cm2 1.25 x 106
100 mm
plate 10 mL 56 cm2 2.8 x 106
60 mm
EmbryoMax® PMEF, Strain DR4, Mytomycin C treated, passage 3
75 cm2 flask 12 mL 75 cm2 3.75 x 106
25 cm2 flask 6 mL 25 cm2 1.25 x 106
100 mm
plate 10 mL 56 cm2 2.8 x 106
60 mm
EmbryoMax® PMEF, Strain DR4, Irradiated, passage 3
75 cm2 flask 12 mL 75 cm2 3.75 x 106
25 cm2 flask 6 mL 25 cm2 1.25 x 106
100 mm
plate 10 mL 56 cm2 2.8 x 106
60 mm
Monoclonal Anti-CD8-Biotin antibody produced in mouse (B0406)
µl of diluent. Incu-
bate at room temperature (18 - 22 °C) for 30 min-
utes. Protect from light at this and all subsequent
steps.
9. a. If whole blood is used, use lysing solution after
incubation
Microsoft Word - VP001 tech bulletin final
allow cells to reach viable cell
density (VCD) greater than 8 106 viable cells/mL
for optimal performance. Discard cells after
30 passages.
3. Subculture cells for at least three passages prior
Product Information Sheet - SHM05
1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0
90-100 mm
dish 15-30 x 10
5 5-10 x 106 150 800 8.0
T-25 flask 5-10 x
Product Information Sheet - SHM03
1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0
90-100 mm
dish 15-30 x 10
5 5-10 x 106 150 800 8.0
T-25 flask 5-10 x
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