106-30-9

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Showing 31-60 of 1336 results for "106-30-9" within Technical Documents

1.0 6 well 2-5 x 105 1-2 x 106 30 200 2.0 60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0 90-100 mm dish 15-30 x 10 5 5-10 x 106 150 800 8.0 T-25 flask 5-10 x

Monoclonal Anti-CD15-Biotin antibody produced in mouse (B0906)

. Product Profile When assayed by flow cytometric analysis, using 10 µl of the antibody per 1 X 106 cells and ExtrAvidin -FITC Conjugate (Product No. E 2761), a fluorescence intensity is observed similar

Product Information Sheet - SHM01

1.0 6 well 2-5 x 105 1-2 x 106 30 200 2.0 60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0 90-100 mm dish 15-30 x 10 5 5-10 x 106 150 800 8.0 T-25 flask 5-10 x

Product Information Sheet - SHM04

1.0 6 well 2-5 x 105 1-2 x 106 30 200 2.0 60 mm dish 5-10 x 105 2.5-5 x 106 60 400 4.0 90-100 mm dish 15-30 x 10 5 5-10 x 106 150 800 8.0 T-25 flask 5-10 x

Monoclonal Anti-B23 antibody produced in mouse (B0556)

µl of diluent. Incu- bate at room temperature (18 - 22 °C) for 30 minutes. Protect from light at this and all subsequent steps. 9. a. If whole blood is used, use lysing solution after incubation

MONOCLONAL ANTI-HUMAN CD16 CLONE BL-LGL/1 Purified Mouse Immunoglobulin

or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) contain- ing cells to be stained. Vortex tube gently. Incu- bate the cells at room temperature (18–22 °C) for

Data Sheet - C6555

stained, i.e. 1 µg of monoclonal antibody per 1 x 106 cells in a final volume of 100 µl. Tap tube gently to mix. Incubate the cells on ice for 30 minutes. Proper controls to

FlowCellect™ Mouse TH1/TH2 Intracellular Cytokine Kit

for 15 minutes in the dark. 9. Spin down cells at 2500 rpm (600Xg) for 3 minutes and discard buffer. 10. Resuspend the cells in 1mL of 1X Assay Buffer/5X 106 cells by gently pipetting up

FlowCellect™ Mouse TH1 Intracellular Cytokine Kit

for 15 minutes in the dark. 9. Spin down cells at 2500 rpm (600Xg) for 3 minutes and discard buffer. 10. Resuspend the cells in 1mL of 1X Assay Buffer/5X 106 cells by gently pipetting up

Product Information Sheet - CS0270

Mix well by tapping the side of the plate gently for 10 seconds. 8. Incubate for 30 minutes at room temperature. 9. Read the optical density (OD) of each well using a multiwell plate reader set

Monoclonal Anti-CD11b antibody produced in mouse (C0551)

stained, i.e. 1 µg of monoclonal antibody per 1 x 106 cells in a final volume of 100 µl. Tap tube gently to mix. Incubate the cells on ice for 30 minutes. Proper controls to be included for

EmbryoMax® PMEF, Strain DR4, Mytomycin C treated, passage 3

75 cm2 flask 12 mL 75 cm2 3.75 x 106 25 cm2 flask 6 mL 25 cm2 1.25 x 106 100 mm plate 10 mL 56 cm2 2.8 x 106 60 mm

Monoclonal Anti-Human CD11b Biotin Conjugate Purified Mouse Immunoglobulin Clone 44

sensitive. Performance When assayed by flow cytometric analysis, using 10 µl of the antibody per 1 X 106 cells and ExtrAvidin®- FITC Conjugate (Sigma Product No. E-2761), a fluo- rescence intensity is observed

MONOCLONAL ANTI-HUMAN CD11C CLONE 3.9 Biotin Conjugate Purified Mouse Immunoglobulin

100 µl of diluent. Incubate at room temperature (18 - 22°C) for 30 minutes. Protect from light at this and all subsequent steps. 9. a. If whole blood is used, use lysing solution after incubation

Considerations for Bioreactor Process Development and Scale-Up

seed the production vessels at a target VCD of ≥ 1.2 x 106 cells/mL. Approximately 22 ± 4 hours post-seeding, when an N-stage VCD of ≥ 2.4 x 106 cells/mL was achieved, the transfection mixture was prepared

Imprint Ultra Chromatin Immunoprecipitation Kit User Guide

requirements (Figs. 7-9 in Appendix). Follow the guidelines indicated in the table below: TF abundance Application Number of cells Histone modification or abundant TF qPCR 0.5 - 2 x 106 Microarray

Constituents and Additives - Colorants

Temperature: 30 °C Diluent (v/v): Methanol and water (50:50) Sample:

Data Sheet - C7798

or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) con- taining cells to be stained. Vortex tube gently. Incu- bate the cells at room temperature (18 – 22 °C) for 30

Data Sheet - C7423

or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) containing cells to be stained. Vortex tube gently. Incubate the cells at room temperature (18 – 22 °C) for 30 minutes.

TB329 Sf9 Insect Cells

Flask Size Cell Number Medium Volume 25-cm2 flask 1.0 × 106 5 ml 75-cm2 flask 3.0 × 106 10 ml 150-cm2 flask 6.0 × 106 30 ml Passaging Suspension Cultures Sf9 Insect

Monoclonal Anti-CD38 antibody produced in mouse (C1586)

1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) contain- ing cells to be stained. Vortex tube gently. Incu- bate the cells at room temperature (18 – 22 °C) for

Product Information Sheet - CS0310

Solution to the medium at a 1:30 ratio. For example, add 10 µL 30X FLICA to 290 µL medium. 7. Mix well. 8. Incubate cells for 1 hour at 37 °C under 5%

Product Information Sheet - CS0300

Solution to the medium at a 1:30 ratio. For example, add 10 µL 30X FLICA to 290 µL medium. 7. Mix well. 8. Incubate cells for 1 hour at 37 o C under

Data Sheet - C7673

1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) containing cells to be stained. Vortex tube gently. Incubate the cells at room temperature (18 to 22

Monoclonal Anti-CD36 antibody produced in mouse (C4679)

or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) containing cells to be stained. Vortex tube gently. Incubate the cells at room temperature (18 – 22 °C) for

Monoclonal Anti-CD4-Biotin antibody produced in mouse (B7280)

100 µl of diluent. Incu- bate at room temperature (18 - 22 °C) for 30 min- utes. Protect from light at this and all subsequent steps. 9. a. If whole blood is used, use lysing solution after incubation

TC RNA Oligonucleotides Synthesis and Application

protected RNA phosphoramidites in the synthesis of oligoribonucleotides with a length of 24, 48 and 106 nucleotides and demonstrates the biological functionality of the resultant products. * D.J.Dellinger

Data Sheet - C7298

or 1 x 106 cells per tube. 2. Add 5 µl of monoclonal antibody to tube(s) containing cells to be stained. Vortex tube gently to mix. Incubate the cells at room temperature (18 - 22 °C) for 30 minutes

A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions

Condition Cell Number per 6-Well Dish HEScGRO Accumax 1.7 x 106 KOSR Collagenase 1.3 x 106 HEScGRO Manual 0.5 x 106 KOSR Manual 1.6 x 106 0.000 0.200 0.400 0.600

Data Sheet - F1672

nine members (FGF-1 to FGF-9), with a 30-50% sequence identity at the amino acid level. All besides FGF-8 contain two conserved positions of two cysteine residues. FGF-9, also known as glia activating

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