1979).
Biddison, W. E., et al., J. Immunol., 131, 152 (1983).
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frequently achieving densities of 4-5
x 106 cells/mL (See Figures 1A, 1B and 1C). The Sf-900 II SFM
and High Five cultures typically had cell densities ranging from
2-3.5 x 106 cells/mL. The HyQ® SFX-Insect
amino acid residue signal peptide that is cleaved to
generate a mature protein predicted to contain 93
amino acid residues with an N-glycosylation site.
Mature human eotaxin-2 has a predicted molecular
106 cells be used for the input. Inputs of up to 5
x 106 cells may be used, however slight cross-
contamination of genomic DNA in the RNA
fraction may be observed in input ranges over
106 cells
Biosafety in
Microbiological and Biomedical Laboratories
Human Health Service Publication No. (CDC) 93-
8395. U.S. Dept. of Health and Human Services;
4th Edition U.S. Government Printing Office
Washington
it is recommended that
1 × 106 cells be used for the input. As a general
guideline, a confluent 3.5 cm plate of HeLa cells will
contain 106 cells. Inputs of up to 5 × 106 cells may be
used;
to 1.0 × 106 cells/ml in culture medium containing 1 μg/ml
actinomycin D.
– Incubate 3 hours at +37°C and 5% CO2 .
Spin cells down and resuspend to a final concentration of 1.0 ×
Cloning of a novel receptor
expressed in rat prostate and ovary. Proc. Natl.
Acad. Sci. U.S.A., 93(12), 5925–30 (1996).
2. Mosselman, S. et al., ER beta: identification and
characterization of a
101
Red Grape Juice 155 158 98
Apple Juice 106 102 104
Orange Juice 90 90 100
Multivitamin Juice 119 119 100
Lemonade 93 91 102
Orangeade 94 92 102
Cola 106 106 100
The measured values match to those