of
variation of fluorescence peaks, and uniformity of
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CHROMATIN IMMUNOPRECIPITATION PROTOCOL 9
A. In vivo Crosslinking and Lysis 9
B. Sonication to Shear DNA 10
C. Immunoprecipitation (IP) of Crosslinked Protein/DNA 11
D. Elution and Reverse Crosslinks
cells, at ~80% confluency, this is approximately 4 x 107 cells. This will
generate a preparation of chromatin that can be used for approximately 11
separate immunoprecipitations given a final concentration
infiltration or
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