is defined as the amount of
cobra venom anti-complementary protein, which causes
50% inhibition of lysis of 2 x 108 antibody sensitized
sheep erythrocytes (Product No. E9383) when
incubated with human
β-mercaptoethanol to 14ml of denaturating solution per
108 cells. This solution can be stored one month at room temperature.
2. Add 10ml of solution D per 108 cells. Mix thoroughly and allow to sit 1 min at
particles with
100 µL of wash buffer (7 mM Tris, pH 8.0, 0.17 M
NaCl) twice.
9. Elute the bound mRNA with 25-50 µL of DEPC
treated water at 55 °C for two minutes.
(1.5 × 108 cells/ml)
(µl)
GVB2+
(µl)
Distilled
water
(µl)
A** v – 200 300–v –
B v 5 200 295–v –
C v 10 200 290–v –
D v 20 200 280–v –
E v 30 200 270–v –
F v
unit is defined as
the amount of cobra venom anti-complementary protein
which causes 50% inhibition of lysis 2 x 108 antibody
sensitized sheep erythrocytes (Sigma Product No.
E7509) when incubated with
dilution required should be
determined by the investigator.
5. Prepare a suspension of 1.5 108 cells/mL of
antibody sensitized sheep erythrocytes in GVB2+.
6. Pipette the diluted complement C8
between 1.67 x 108 and
2.30 x 108 CFU/disk and retentive
filters had LRVs reported as “>8.22”
to “>8.36”. The data from these
experiments are found in Table 1.
47 10.1 3.72 115 55 13.6 7.45 108
46 10.3
Control Result shows
Transformation of uncut vector DNA, such as 50 pg. Transformation efficiency of cells per microgram of DNA
>1 × 108 .
Transformation of linearized unligated vector DNA. Completeness
glycerol.
Molecular mass: ∼142 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 80–108 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for