glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
J., et al., The growth of cells in serum-
free hormone-supplemented media. Methods
Enzymol. 58 94-109 (1979).
CMH/RXR 12/03
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
Standard Curve
200 µM t-BuOOH
Solution (µl)
nmoles per
reaction volume
0 0
5 1
10 2
20 4
40 8
60 12
80 16
2. Place up to 100 µl of test sample in a tube. Bring
the final
.5 h 6 h 16 h 24 h
Figure 2. DNA damage/Genotoxicity Dose Response in HEPG2 Cells
Figure 4. DNA damage/Genotoxicity Time Course in HEPG2 Cells
Color
Fold
Change