I HADK2-109 4689 - 9739 pg/mL
Control II HADK2-209 21943 - 45574 pg/mL
IL-8 Control I HADK2-109 70 - 145 pg/mL
Control II HADK2-209 302 - 626 pg/mL
MCP-1 Control
0.1 mM
PMSF, and 25% glycerol.
Molecular mass: ∼79 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
on dry ice and storage at –70 C is
recommended. Repeated freezing and thawing is not
recommended.
References
1. Kidmose, R.T. et al., Proc. Nat. Acad. Sci. USA,
109(38), 15425-15430 (2012).
supply chain concerns
• Proven process performance at 3 L scale with
genome titer exceeding 8 x 109 gc/mL
• Comprehensive user protocols to guide from
seed train through at-scale transfection and
1. Pellet yeast cells (2 x 109 cells) from mid-log phase
(1-5 x 107 cells/ml) by centrifugation at
3,000−5,000 x g for 5 minutes.
2. Resuspend the pellet with 4−6 volumes of distilled
water (5
recombinant human TRAIL solution should be
stored in aliquots at −70 °C. Avoid multiple freeze-thaw
cycles. It is stable for at least 6 months at this
temperature.
Product Profile
Recombinant human
at −20 °C or −70 °C. Reconstituted
product may be stored at 2-8 °C for up to one month.
For prolonged storage, the product may be frozen in
working aliquots at −20 °C to −70 °C for 6 months
without
remain susceptible to viral infection, and
in this study, Canine Adenovirus (CAV) titers in excess of 109
infectious particles/mL were obtained.
Materials
Cells and Virus
• Madin Darby Canine Kidney
molecular mass of the
monomer is 109 kDa, but as a result of glycosylation,
the recombinant monomer migrates at >150 kDa on
reducing SDS-PAGE.1,2
Oncostatin M is a member of the IL-6-type family of
cytokines
Interaction, New York: Springer-Verlag,
p. 206 (1979).
8. Orr-Urtreger, A., et al., Development, 109,
911(1990).
9. Manova, K., et al., Development, 110, 1057 (1990).
10. Arakawa, T., et al., J.
up to 12 months after date of receipt. After
reconstitution maintain at -20°C to -70°C in undiluted aliquots for up to 6 months. Avoid
repeated freeze/thaw cycles. Glycerol (ASC grade or better) can be
toward various aglycones
using uridine diphospho[U-14C] glucuronic acid.
Anal. Biochem., 109, 321-329 (1980).
6. Strassburg, C. P., et al., Expression of the UDP-
glucuronosyltransferase 1A Locus in
toward various aglycones
using uridine diphospho[U-14C] glucuronic acid.
Anal. Biochem., 109, 321-329 (1980).
6. Strassburg, C. P., et al., Expression of the UDP-
glucuronosyltransferase 1A Locus in
,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
Res., 206, 261-275 (1993)
5. Sage, H. et al., J. Cell Physiol., 127, 373-387 (1986)
6. Sage, H. et al., J. Cell Biol., 109, 341-356 (1989)
7. a. Hasselaar, P. et al., J. Biol. Chem., 266,
13278-13184
(1985).
16. Barnes, D., Meth. Enzymol., 163, 707 (1988).
17. Pierce, G., et al., J. Cell Biol., 109, 429 (1989).
18. Barrett, T., and Benditt, E., Proc. Natl. Acad. Sci.
USA, 85, 2810 (1988).
19
(1985).
16. Barnes, D., Meth. Enzymol., 163, 707 (1988).
17. Pierce, G., et al., J. Cell Biol., 109, 429 (1989).
18. Barrett, T., and Benditt, E., Proc. Natl. Acad. Sci.
USA, 85, 2810 (1988).