I HADK2-109 4689 - 9739 pg/mL
Control II HADK2-209 21943 - 45574 pg/mL
IL-8 Control I HADK2-109 70 - 145 pg/mL
Control II HADK2-209 302 - 626 pg/mL
MCP-1 Control
0.1 mM
PMSF, and 25% glycerol.
Molecular mass: ∼79 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
on dry ice and storage at –70 C is
recommended. Repeated freezing and thawing is not
recommended.
References
1. Kidmose, R.T. et al., Proc. Nat. Acad. Sci. USA,
109(38), 15425-15430 (2012).
supply chain concerns
• Proven process performance at 3 L scale with
genome titer exceeding 8 x 109 gc/mL
• Comprehensive user protocols to guide from
seed train through at-scale transfection and
productivity rates are listed in Table 6.
Table 6. Performance criteria and productivity control strains according to ISO 9308-1:2014 (table 1)
The recovery rate of 70 % is equivalent to a productivity
1. Pellet yeast cells (2 x 109 cells) from mid-log phase
(1-5 x 107 cells/ml) by centrifugation at
3,000−5,000 x g for 5 minutes.
2. Resuspend the pellet with 4−6 volumes of distilled
water (5
recombinant human TRAIL solution should be
stored in aliquots at −70 °C. Avoid multiple freeze-thaw
cycles. It is stable for at least 6 months at this
temperature.
Product Profile
Recombinant human
at −20 °C or −70 °C. Reconstituted
product may be stored at 2-8 °C for up to one month.
For prolonged storage, the product may be frozen in
working aliquots at −20 °C to −70 °C for 6 months
without
remain susceptible to viral infection, and
in this study, Canine Adenovirus (CAV) titers in excess of 109
infectious particles/mL were obtained.
Materials
Cells and Virus
• Madin Darby Canine Kidney
molecular mass of the
monomer is 109 kDa, but as a result of glycosylation,
the recombinant monomer migrates at >150 kDa on
reducing SDS-PAGE.1,2
Oncostatin M is a member of the IL-6-type family of
cytokines
Interaction, New York: Springer-Verlag,
p. 206 (1979).
8. Orr-Urtreger, A., et al., Development, 109,
911(1990).
9. Manova, K., et al., Development, 110, 1057 (1990).
10. Arakawa, T., et al., J.
up to 12 months after date of receipt. After
reconstitution maintain at -20°C to -70°C in undiluted aliquots for up to 6 months. Avoid
repeated freeze/thaw cycles. Glycerol (ASC grade or better) can be
toward various aglycones
using uridine diphospho[U-14C] glucuronic acid.
Anal. Biochem., 109, 321-329 (1980).
6. Strassburg, C. P., et al., Expression of the UDP-
glucuronosyltransferase 1A Locus in
toward various aglycones
using uridine diphospho[U-14C] glucuronic acid.
Anal. Biochem., 109, 321-329 (1980).
6. Strassburg, C. P., et al., Expression of the UDP-
glucuronosyltransferase 1A Locus in
,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product