cells. The control with no
addition was 1. Weiss, A., et al., J. Immunol., 133, 123 (1984)
2. Manger, B., et al., J. Clin. Invest. 77, 1501 (1986)
JWM/2002
peptide cor-
responding to amino acids 119-133 of human
caspase 4 protein, conjugated to KLH. The isotype is
determined using Sigma ImmunoTypeTM Kit (Sigma
ISO-1) and by a double diffusion immunoassay using
and
composition."
13. Ross, S.E., et al., BioTechniques, 4, 350-354 (1986).
REVIEW ARTICLES / ADDITIONAL REFERENCES:
Green, N. M., Advances in Protein Chemistry, 29, 85-133 (1975).
"Avidin."
Reactions (Jacobson, M. K., and Jacobson, E. L.,
eds.). Springer-Verlag Press (New York),
pp. 130-133 (1989).
5. Stoica, B. A. et al., J. Forensic Sci., 61(5),
1331-1336 (2016).
6. Park, A.-M
1479, (1995).
12. Sastry, C.S.P. and Lingeswara Rao, J.S.V.M.,
Indian J. Pharm. Sci. 57, 133, (1995).
13. Berthou, F. and Dreano, Y., J. Chromatogr. 616,
117, (1993).
14. Weir, P.J. et al., J.
vs. EB1b)
and 47% (EB1a/b vs. EB1c) overall amino acid
sequence similarity.5-8 The N-termini (1-133 amino
acids) of EB1a and EB1b are ~93% and ~73% similar,
respectively, to the N-terminus of EB1c
hours
at 50 °C and for at least 24 hours at –20 °C.
References
1. Thelander, L., and Reichard, P., Annu. Rev.
Biochem., 48, 133-158 (1979).
2. Holmgreen, A., Annu. Rev. Biochem., 54, 237-271
(
in water (133 mg/ml), yielding a
clear, colorless solution. The pH of aqueous KI
solutions is neutral to slightly alkaline. This product is
also soluble in alcohol, glycerol, and glycol.1
Storage
Greenberg, M.E., Cell, 79, 5-8
(1994).
6. Yin, J.C.P., et al., Cell, 81, 107-115 (1995).
SC,PHC 05/13-1
2013 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
EB1b)
and 47% (EB1a/b vs. EB1c) overall amino acid
sequence similarity.5-8 The N-termini (1-133 amino
acids) of EB1a and EB1b are ~93% and ~73% similar,
respectively, to the N-terminus of EB1c
pH 1. However, when the pH
was raised to pH 3, avidin regained native configuration
and binding activity. The complex is also extremely
stable at high pH, being only 20% ionized even at
pH 13.
dehydrated powder, in a dry place,
in tightly-sealed containers at 2-25°C.
Directions:
Add 13 g to 1 litre of distilled water. Mix well and distribute into containers fitted with Durham tubes.
Sterilize
1993).
3. Mulligan, C. N., Environmental applications for
biosurfactants. Env. Pol., 133, 183-198 (2005).
4. Hoornstra, D. et al., A new method for in vitro
detection of microbially produced
caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic Press, New York
6. (1975).
7. S.C. Gill and P.H. von Hippel, Anal. Biochem., 182, 319
References
1. Merck Index 12, 5011 (1996).
2. Chance, RE and Frank, BH. Research,
Development, Production, and Safety of
Biosynthetic Human Insulin, Diabetes Care 16
(Suppl 3), 133-142 (1993).
References
1. Merck Index 12, 5011 (1996).
2. Chance, RE and Frank, BH. Research,
Development, Production, and Safety of
Biosynthetic Human Insulin, Diabetes Care 16
(Suppl 3), 133-142 (1993).
molds any mycoplasm.
References
1. Smith, K., Science, 240, 1169 (1988).
2. Morgan, D., et al., Science, 193, 1007 (1976).
3. Ortaldo, J., et al., J. Immunol., 133, 779 (1984).
4. Farrar, J., et al.
and mycoplasm.
References
1. Smith, K., Science, 240, 1169 (1988).
2. Morgan, D., et al., Science, 193, 1007 (1976).
3. Ortaldo, J., et al., J. Immunol., 133, 779 (1984).
4. Farrar, J., et
1985).
11. Björck, L., and Kronvall, G., J. Immunol., 133, 969
(1984).
12. De Chateau, M., et al., Scand. J. Immunol., 37(4),
399 (1993).
13. It should be noted that protein L is restricted to
capillary zone electrophoresis-
electrospray ionization mass spectrometry. J.
Chromatogr. A, 988(1), 127-133 (2003).
GCY/JRC 8/03
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
# ...................... MP4500000
CAS No. ..................... 17455-13-9
LITERATURE REFERENCES:
Fieser 4, 142; 5, 152; 6, 133; 7, 76; 8, 128; 9, 126
"Crown ethers" is the common name for a group