column to the top with
buffer. Allow ~5 CV of buffer to drain through the
bed at a flow rate at least 133% of the flow rate
to be used in the procedure. The bed height
should have
glycerol.
Molecular mass: ∼112 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 133–181 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not
glycerol.
Molecular mass: ∼112 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 133–181 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not
glycerol.
Molecular mass: ∼112 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 133–181 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not
column to the top with
buffer. Allow ~5 CV of buffer to drain through the
bed at a flow rate at least 133% of the flow rate
to be used in the procedure. The bed height
should have
in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM
MgCl2, 1 mM dithioerythritol, 1 mM ATP, pH 7.5
(at 20°C) resulting in >90 % recovery of 1 µg pBR322
DNA×
Enhanced
chemiluminescent reactions catalyzed by
horseradish peroxidase. Methods Enzymol., 133,
331-353 (1986).
4. Archer, S.L. et al., Detection of activated O2
species in vitro and in rat lungs by
incubation for 16 h at 4° C in 66 mM
Tris-HCl, 5 mM MgCl2, 5 mM Dithioerythritol, 1 mM
ATP, pH 7.5 (at 20°C) resulting in >90 % recovery of
λDNA.
Subsequent re-cutting with Asp718 I yields > 90%
16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM dithiothreitol, 1 mM ATP, pH 7.5 (at 20° C) result-
ing in >90 % recovery of 1 μg �DNA fragments.
Subsequent re-cutting with Nsi I yields >
for 16 h at +4 °C in 66 mM
Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP,
pH 7.5 (at +20 °C), resulting in >90 % recovery of Ad2
DNA.
Subsequent re-cutting with Nar I yields > 90%
Phosphatase
Dephosphorylation of
5´-phosphate residues
from nucleic acids.
1,000 U
5,000 U
04 898 133 001
04 898 141 001
Rapid DNA
Ligation Kit
Ligation of nucleic acids. Kit
(40 DNA ligations)
Glycosciences: status & perspectives”, H.-J. Gabius & S. Gabius
(Eds.), Chapman & Hall, Weinheim, 1997, pp. 133.
14. J. Kihlberg in “Fmoc solid phase peptide synthesis: a practical approach”, W. C. Chan &
P.
the marsupial brushtail
possum (Trichosurus vulpecula).
Janine A Duckworth et. al
Reproduction, 133 (1), 177-186 (2007)
In a previous study, three infertility-relevant epitopes of possum ZP2 (Pep12
heat up, there were temperature delays in
the system and although the temperature stabilized
at 133-135 ºC above the 121 ºC threshold, there
was ~1 ºC temperature difference across the
prefilter during
Cardiol. 39(1): 133-147.
2. Litzkas, P., Jha, K.K., Ozer, H.L. (1984) Efficient transfer of
cloned DNA into human diploid cells: protoplast fusion in
suspension. Mol. Cell. Biol 4(11): 2549-2552
in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at 20° C) result-
ing in >90 % recovery of 1 μg pBR322
h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM
Dithiothreitol, 1 mM ATP, pH 7.5 (at 20°C) resulting in
>80% recovery of 1 μg �DNA × Pvu II fragments.
Subsequent re-cutting with Xho I yields > 90%
ligase
in a volume of 10 �l by incubation for 16 h at 4°C in 66
mM Tris-HCl, 5 mM MgCl2, 5 mM dithiothreitol, 1 mM
ATP, pH 7.5 (at 20°C) resulting in >90 % recovery of
� dcm–-DNA.
Subsequent