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3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

Thermal Transitions of Homopolymers: Glass Transition & Melting Point

Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.

Detection, identification, differentiation and cultivation of Yersinia species

Garg Group – Professor Product Portal

The Garg group develops methods for the synthesis of natural products and pharmaceuticals. One key method pertains to heterocyclic arynes, such as indolynes and pyridynes, which are generated in situ from silyltriflate precursors under mild fluoride based reaction conditions.

Authenticated Breast Cancer Cell Lines for Cancer Research

Choose from more than 30 breast cancer cell lines authenticated by ECACC, classified by hormone receptor-positive, HER2-positive, and triple-negative cancers, as well as by known mutations such as BRCA2.

Forskolin-Induced Swelling Assays

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Phosgene and Phosgene Substitutes

Phosgene is 170 times more reactive than TP, the main phosgene substitute. Therefore, reactions with phosgene can be carried out under much milder conditions than with TP. Compounds will react faster and at lower temperatures (often at -78°C), preserving sensitive

radiello® Diffusive Air Sampling Application - Volatile Organic Compounds (VOCs) Chemically Desorbed With CS2

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