cold EX-CELLTM 302 medium.
3. Using low-speed centrifugation, pellet the cell suspension
at 200 g for 5 minutes and carefully decant the supernatant
without disturbing the cell pellet.
4. Resuspend the
cold EX-CELLTM 302 medium.
3. Using low-speed centrifugation, pellet the cell suspension
at 200 g for 5 minutes and carefully decant the supernatant
without disturbing the cell pellet.
4. Resuspend the
EX-CELL® 302 is formulated with sodium bicarbonate
and without L-glutamine. For applications requiring the
use of L-glutamine, supplement with 4 mM L-glutamine
by adding 20 mL/L of a 200 mM solution
EX-CELL® 302 is formulated with sodium bicarbonate
and without L-glutamine. For applications requiring the
use of L-glutamine, supplement with 4 mM L-glutamine
by adding 20 mL/L of a 200 mM solution
use, all media were supplemented with the
following concentrations of L-glutamine:
• EX-CELLTM 302 — 4 mM
• EX-CELLTM 293 — 6 mM
• EX-CELLTM VPRO — 6 mM
All cultures were seeded at 3 x105 cells/mL, in
selection system we recommend the addition of
4 mM L-glutamine for optimal growth.
Due to current regulatory concerns about the sources of raw
materials, EX-CELLTM 302 was developed using only
recombinant human
L-glutamine. For applications
requiring the use of L-glutamine, supplement with
4 mM L-glutamine prior to use by adding 20 mL/L of a
200 mM solution (Catalog No. 59202C). SAFC Biosciences
recommends L-glutamine
gas.
NOTE: For applications requiring the use of L-glutamine,
supplement with 4 mM L-glutamine by adding 20 mL/L of
a 200 mM solution (Catalog No. 59202C) prior to use.
SAFC Biosciences recommends L-glutamine
, in a mixture of Diluent and water (1:4)
Sample solution: Transfer a portion of the powdered pellets (about 80–90 mg), from the Capsule content,
to a 200-mL volumetric flask, add 20 mL of methanol
Cast the gel and allow it to solidify.
4. Load samples and run the gels using your standard protocol.
5. View the stained gel using a standard transilluminator (302 or 312 nm) and image the gel using an
precast gels at 4°C can cause dye precipitation
and poor performance.
4. Load samples and run the gels using your standard protocol.
5. View the stained gel with a standard transilluminator (302 or 312 nm)
Cast the gel and allow it to solidify.
4. Load samples and run the gels using your standard protocol.
5. View the stained gel using a standard transilluminator (302 or 312 nm) and image the gel using an
precast gels at 4°C can cause dye precipitation
and poor performance.
4. Load samples and run the gels using your standard protocol.
5. View the stained gel with a standard transilluminator (302 or 312 nm)
Save the supernatant for
application.
Control
Suggested Positive Antigen Control: Cat.#
12-302, EGF-stimulated A431 cell lysate is
provided as a free positive antigen control for
western immunoblotting
11397
Media and Reagents
• EX-CELL 302 Serum-Free Medium for CHO Cells, SAFC,
Catalog No. 14324C
• EX-CELL 66131, SAFC, Product No. 66131C
• L-Glutamine, 200 mM solution, Sigma-Aldrich, Catalog
and Cline HT., Trends Neurosci., 30,
382-9 (2007).
10. Nigam R., et al., Arch Dermatol Res., 302,
507-15 (2010).
11. Watanabe M., et al., Int Rev Cytol., 213, 1-47
(2002).
12. Ring H.
0.16 (0.353)
Design Temperature
Short Term Use*
Max (Dry Heat) 200 °C (392 °F)
Max (Steam) 150 °C (302 °F)
Min (-)20 °C ((-)4 °F)
Additional Information
Material The Diaphragm is manufactured
6. Pei, L., Proc. Natl. Acad. Sci. USA, 100, 7803-7807
(2003).
7. Mu, D., Cancer Cell, 3, 297-302 (2003).
8. Lauritzen, I. et al., J. Biol. Chem., 278, 32068-
32076(2003)
MCT/PHC 08/04
Director, Commercial Development; DuPont
Experimental Station E268; 200 Powdermill Rd.; Wilmington,
DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax),
licensing@dupont.com.
SILu is a trademark
) –1.0 x 10–13
Torr at 200°C (392°F) –1.0 x 10–5
kPa at 20°C (68°F) –1.0 x 10–14
kPa at 200°C (392°F) –1.0 x 10–6
Evaporation Loss
4 x 10–6 torr at 150°C
Director, Commercial
Development; DuPont Experimental Station E268;
200 Powdermill Rd.; Wilmington, DE 19803; 1-877-
881- 9787 (voice), 1-302-695-1437 (fax),
licensing@dupont.com.
SILu is a trademark of
Director, Commercial
Development; DuPont Experimental Station E268; 200
Powdermill Rd.; Wilmington, DE 19803; 1-877-881-
9787 (voice), 1-302-695-1437 (fax),
licensing@dupont.com.
SILu is a trademark of
Director, Commercial
Development; DuPont Experimental Station E268;
200 Powdermill Rd.; Wilmington, DE 19803; 1-877-
881- 9787 (voice), 1-302-695-1437 (fax),
licensing@dupont.com.
SILu is a trademark of
This is particularly striking
considering the nervous system of C. elegans is
comprised of only 302 neurons. In mammals, 9 different
4TM/2P K+ channels, designated as the KCNK family,
have been cloned
most diverse group of K+
channels in this organism.4 This is particularly striking
considering the nervous system of C. elegans is
comprised of only 302 neurons. In mammals, 9 different
4TM/2P K+ channels