200-580-7

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High-resolution liquid chromatography of fluorescent dye-labeled nucleic acids.

P J Oefner et al.

Analytical biochemistry, 223(1), 39-46 (1994-11-15)

Using 100 mM of triethylammonium acetate as ion-pairing reagent, phosphodiester oligonucleotides labeled fluorescently at their 5' terminus could be separated successfully on alkylated nonporous 2.3-microns poly(styrene-divinylbenzene) particles by means of high-resolution liquid chromatography. Applying excitation wavelengths of 490, 520, 550

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