inspection.
The Separate Source standards are written into many contracts,
specified in US EPA SW-846, and as part of the accreditation by
The NELAC Institute (TNI). Separate Source standards are required
001
Blocking Reagent 50 g 11 096 176 001
Anti-Digoxigenin-AP, Fab fragments 150 U, 200 μl 11 093 274 910
1 2 3
1 2 3
https://www.sigmaaldrich.com
Pa
pe
r
Hybridization Bags 50 bags, 25 cm x 23 cm 11 666 649 001
Reagents, kits
Tris hydrochloride 500 g 10 812 846 001
Buffers in a Box, Premixed SSC Buffer, 20x 4 l 11 666 681 001
Sodium Dodecyl Sulfate (SDS) 1 kg
blocking solution)
Dissolve 2 g of skim milk powder in 200 ml of
TBST with stirring for at least 30 minutes.
Primary antibody • Centrifuge primary antibody for 2 minutes
at 5,000 to 10,000 × g.
methods to elute tagged protein:
Method Step
EDTA elution Add 1 to 2 matrix volumes (50 to 200 µl) of EDTA elution buffer.
– Rock at +2 to +8°C for 15 to 30 minutes.
– Pellet matrix, saving
week
at +2 to
+8°C
Anti-
body
dilution
and
blocking
of mem-
brane
Skim milk:
Dissolve 2 g skim milk powder in
200 ml of TBST with stirring for at
least 30 min.
1 week
at +2 to
+8°C
1998) Multiple Roles for Hepatocyte Growth Factor in Sympathetic Neuron
Development. Neuron 20:835-846.
Matter, M. L., Z. Zhang, C. Nordstedt, and E. Ruoslahti. (1998) The alpha5ß1
Integrin Mediates
1998) Multiple Roles for Hepatocyte Growth Factor in Sympathetic Neuron
Development. Neuron 20:835-846.
Matter, M. L., Z. Zhang, C. Nordstedt, and E. Ruoslahti. (1998) The
alpha5ß1 Integrin Mediates
1998) Multiple Roles for Hepatocyte Growth Factor in Sympathetic Neuron
Development. Neuron 20:835-846.
Matter, M. L., Z. Zhang, C. Nordstedt, and E. Ruoslahti. (1998) The
alpha5ß1 Integrin Mediates