on frozen sections using
PAP (Peroxidase Anti-Peroxidase) detection is
1:2,000-1:4,000 . Dilution in the range of 1:200-1:400 is
recommended for immunofluorescent detection on
frozen sections.
Becq, F. et al., J. Biol. Chem., 271, 16171-16179
(1996).
16. Thienpoint, D. et al., Nature, 209, 1084-1086
(1966).
17. Rousseau, R. et al., Eur. J. Drug Met.
Pharmacokinet., 6, 281-288
we
recommend determining optimal working dilutions by
titration.
References
1. Lee, R.T., et al., Cir. Res., 76, 209 (1995).
2. Chan, B.M., et al., Cell, 68, 1051 (1992).
3. Bergelson, J.M., et al
cytosolic fraction of rat brain using 2 to 3 µg of the
antibody.
A minimum working dilution of 1:400 is determined by
indirect immunoperoxidase staining of trypsin-digested,
formalin-fixed, paraffin-embedded
Ligand/ TRANCE
product, generated after removal of the 16 amino acid
residue signal peptide, contains 209 amino acids with a
calculated mass of 23 kDa. Due to glycosylation, the
recombinant protein migrates
sheets, 20 x 30 cm 11 209 272 001
20 sheets, 10 x 15 cm 11 209 299 001
1 roll, 0.3 x 3 m 11 417 240 001
High Pure Plasmid Isolation Kit 1 kit, 50 purifications 11