positively charged 10 sheets, 20 x 30 cm 11 209 272 001
20 sheets, 10 x 15 cm 11 209 299 001
1 roll, 0.3 x 3 m 11 417 240 001
DIG Wash and Block Buffer Set 1
transfer to a
new DNA low binding tube for each washing step.
9. Add T4 DNA ligase (400 units final volume) with 1 mM ATP and NEB2 buffer to a final volume of 20 µl,
then allow to
with
sealer and incubate at room temperature for 1 hour on an orbital micro-
titer plate shaker set to rotate at moderate speed, approximately 400-500
rpm.
10. Remove plate sealer
Lysates can be stored at – 70 °C.
7. Under these conditions, cell extract dilutions from
1:10 to 1:400 in Standard Diluent Buffer are
sufficient for detection of Tau in this ELISA.
Homogenization
Total RNA x μl 1 to 20 μg
mRNA x μl 0.3 to 2 μg
Oligo[(dT)24 T7 promotor]65 primer 100 pmol/μl (Vial 6)
or
Oligo(dT)15 primer 200 pmol/μl (Vial 5)
2 μl
2 μl
200 pmol
400 pmol
Water
Total RNA x μl 1 to 20 μg
mRNA x μl 0.3 to 2 μg
Oligo[(dT)24 T7 promotor]65 primer 100 pmol/μl (Vial 6)
or
Oligo(dT)15 primer 200 pmol/μl (Vial 5)
2 μl
2 μl
200 pmol
400 pmol
Water
.
Site 1 Results for detection of CMV pp65 antigenemia in all specimens:
Comparative Device
+ - Total
Light Diagnostics™
CMV pp65 IFA Kit
+ 83 9 92
- 8 200 208
pSer199] per
ng Tau
References
1. Liu, F., et al., Involvement of aberrant glycosylation
in phosphorylation of tau by CDK5 and GSK-3β.,
FEBS Lett., 530, 209-214. (2002).
2. Sawamura, N.