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miR-214-3p Hu-Cy3 SmartFlare™ RNA Detection Probe
is
representative.
miR-214-3p Hu-Cy3
SmartFlare™
RNA Detection Probe
Cat. # SF-1618
pack size: 50µL (250
miR-214-3p Hu-Cy5 SmartFlare™ RNA Detection Probe
is representative.
miR-214-3p Hu-Cy5
SmartFlare™
RNA Detection Probe
Cat. # SF-1306
pack size: 50µL (250
Aldrich Polymer Products Application - Transitions
Information
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Repeating Unit Tg (°C) Tm (°C)
Acenaphthylene 214
Acetaldehyde -32 165
4-Acetoxystyrene 116
Acrylamide 165
Acrylic acid 105
Acrylonitrile, syndiotactic
Cell Culture Poster 4
Initially
the absorbance at 214 nm was monitored because it is generally
accepted as the standard peptide-mapping wavelength.5 Figure 2A
shows the TFA chromatograms at 214 nm obtained for two lots of
wheat
Ibuprofen and Codeine MM
1.52022.0001)
Injection: 10 µL
Detection:
Bambuterol and Related Substances (EP)
STAR RP 18 endcapped (5 μm) Hibar® RT 150x4 6 mm 1 51455 0001Column: Purospher® STAR RP-18 endcapped (5 μm), Hibar® RT 150x4.6 mm 1.51455.0001
Injection: 20 μL
Detection: UV, 214 nm
Cell: 10 μL
Flow Rate
Data Sheet - D3162 - Lot 118H88021
5000
6000
m/z
Abundance
51 63
77
89
103
111 126
138
151
163 178
193
207 214
228
242
270
Mattson Galaxy 3020 FT-IR
Product Information Sheet - G4410
fluorometric assay for the
measurement of nitrite in biological samples. Anal.
Biochem., 214(1), 11-16 (1993).
5. Grisham, M. B., et al., Quantitation of nitrate and
nitrite in extracellular fluids.
Continuous Manufacturing - how to overcome feeding challenge of Magnesium stearate
]
Compression force [kN]
5 20 25 30 351510
Excipient System LubMannitol+API traditional mix Mannitol+API+MST
Filler Lubricant
50
100
150
200
250
0
Ta
b
le
t
H
a
rd
Data Sheet - I2535
Radioactivity (SR) of ATP (cpm/nmole)
SR = cpm of 5 µl of γ-32P-ATP Assay Cocktail
nmole of ATP
cpm – value from control (step 7)
nmole – 1.25 nmole (5 µl of 250 µM ATP
Assay Cocktail)
2. Specific
Data Sheet - I2535 - Lot SLBB2672V
Radioactivity (SR) of ATP (cpm/nmole)
SR = cpm of 5 µl of γ-32P-ATP Assay Cocktail
nmole of ATP
cpm – value from control (step 7)
nmole – 1.25 nmole (5 µl of 250 µM ATP
Assay Cocktail)
2. Specific
Data Sheet - I2535 - Lot 129K0847
Radioactivity (SR) of ATP (cpm/nmole)
SR = cpm of 5 µl of γ-32P-ATP Assay Cocktail
nmole of ATP
cpm – value from control (step 7)
nmole – 1.25 nmole (5 µl of 250 µM ATP
Assay Cocktail)
2. Specific
Data Sheet - I2535 - Lot SLBB7291V
Radioactivity (SR) of ATP (cpm/nmole)
SR = cpm of 5 µl of γ-32P-ATP Assay Cocktail
nmole of ATP
cpm – value from control (step 7)
nmole – 1.25 nmole (5 µl of 250 µM ATP
Assay Cocktail)
2. Specific
Data Sheet - I2535 - Lot 109K0723
Radioactivity (SR) of ATP (cpm/nmole)
SR = cpm of 5 µl of γ-32P-ATP Assay Cocktail
nmole of ATP
cpm – value from control (step 7)
nmole – 1.25 nmole (5 µl of 250 µM ATP
Assay Cocktail)
2. Specific
Product Information Sheet - T3383
Formula weight: 480.9 (anhydrous)
pKa = 3.3, 7.7, 9.7 at 25 °C
1
Melting Point: Decomposes at 214 °C2
EMm (220nm) = 13 (free base in 0.1 M HCl)2
EmM (268nm) = 18.04
Supelco SLB -IL60 Ionic Liquid GC Columns
35)
Column Isothermal Programmed
PEG 1 280 °C 280 °C
PEG 2 260 °C 270 °C
PEG 3 250 °C 260 °C
PEG 4 250 °C 260 °C
PEG 5 280 °C 300 °C
SLB-IL60 300 °C 300 °C
Description Cat. No.
SLB-IL60, 15 m
Samplicity® G2 Filtration System with Millex Samplicity® and Millex Filters® User Guide
SAMP2GVNB 250
SAMP2GVNK 1000 (4 × 250/pk)
0.45 µm hydrophilic PVDF filter SAMP2HVNB 250
SAMP2HVNK 1000 (4 × 250/pk)
0.20 µm hydrophilic nylon filter SAMP2GNNB 250
SAMP2GNNK 1000 (4 × 250/pk)
0.45
Data Sheet - I2535 - Lot 019K0647
Radioactivity (SR) of ATP (cpm/nmole)
SR = cpm of 5 µl of γ-32P-ATP Assay Cocktail
nmole of ATP
cpm – value from control (step 7)
nmole – 1.25 nmole (5 µl of 250 µM ATP
Assay Cocktail)
2. Specific
Data Sheet - I2535 - Lot 070M0849
Radioactivity (SR) of ATP (cpm/nmole)
SR = cpm of 5 µl of γ-32P-ATP Assay Cocktail
nmole of ATP
cpm – value from control (step 7)
nmole – 1.25 nmole (5 µl of 250 µM ATP
Assay Cocktail)
2. Specific
Data Sheet - I2535 - Lot 041M0986
Radioactivity (SR) of ATP (cpm/nmole)
SR = cpm of 5 µl of γ-32P-ATP Assay Cocktail
nmole of ATP
cpm – value from control (step 7)
nmole – 1.25 nmole (5 µl of 250 µM ATP
Assay Cocktail)
2. Specific
Product Information Sheet - PP0500
Discovery C18
column (25 cm × 4.6 mm, 5 micron, Catalog Number
504971) using a 20 minute linear gradient from 5–50%
B at 0.7 ml/min with UV detection at 214 nm followed by
mass spectrometry.
Solvent
(T6133) - Enzyme Assay
REFERENCE:
de la Haba, G., Leder, I.G., and Racker, E. (1955) Journal of Biological Chemistry 214, 409-426
NOTES:
1. This assay is based on the cited reference.
2. Triosephosphate Isomerase
Data Sheet - R4904
D’Arcangelo, G., and Curran, T., J. Neurosci., 17,
23-31 (1997).
5. Lambert de Rouvroit, C., et al., Exp. Neurol., 156,
214-217 (1999).
6. Jossin, Y., et al., Cereb. Cortex, 13, 627-633
(2003
Data Sheet - H0164
Barratt, M.J., et al., Proc. Natl. Acad. Sci. USA, 91,
4781-4785 (1994).
5. Thomson, S., et al., Semin. Cell. Biol., 10, 205-214
(1999).
6. Strahl, B.D., et al., Proc. Natl. Acad. Sci. USA, 96
Product Flyer: Chromolith® WP 300 Epoxy 2 mm I.D. Monolithic Silica HPLC Columns Create your own Column Chemistry
Flow rate 2.0 mL/min
Detection 214 nm
Temperature 25 °C
Injection volume 5 µL
Gradient Time %A %B
0 100 0
1 100 0
1.25 0 100
3.5 0 100
3.6 100 0
5 100 0
0 1 2 3 4 5
Time
Product Information Sheet - A9264
, K., Eur. J. Biochem., 53, 481-486 (1975).
5. Yamazaki, Y. et al., Eur. J. Biochem., 77, 511-520 (1977).
6. Haystead, C.M.M. et al., Eur. J. Biochem., 214, 459-467 (1993).
7. Bar-Tana, J. and Cleland
Data Sheet - M7395
temperature 7.5 ± 0.3
[with sodium bicarbonate]
Osmolality 250 mOsm/kg H2O ± 5%
[without sodium bicarbonate]
Osmolality 290 mOsm/kg H2O ± 5%
[with sodium bicarbonate]
Amino Acid Analysis Analysis
PharmPrep®P Sorbent
Dimensions 250 x 4.6 mm
20 µm
10 µm
Column dimension:
50 mm I.D:, 220 mm length
Flow rate: 23.5 CV/h
5
Figure 5.
Packing of PharmPrep™ P 100 RP-8e, 10 µm sorbent into
Product Flyer: Chromolith® WP 300 RP-18 2 mm I.D. Monolithic Silica HPLC Columns - Rapid and Robust Protein Chromatography
0.1% (v/v) TFA
Gradient: Time %A %B
0 5 95
25.0 30 70
30.0 30 70
Flow rate: 0.190 mL/min
Pressure: 18 bar
Detection: Vanquish DAD 20 Hz, UV, 214 nm
Detector cell: LightPipe 10 mm
Temperature
HPLC Certified Millex®-LG and Millex®-LH 4, 13, and 25 mm Syringe Filters User Guide
showed no peaks greater in intensity than 0.004 AUFS (after the
column frontal volume) at either 214 nm or 254 nm.
User Guide
HPLC Certified
Millex®-LG and Millex®-LH
4, 13, and 25 mm Syringe
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