pT7-FLAG-2 Features
Feature Map Position
T7 Promoter 72-91
lacO 92-111
Recommended 5’ primer
sequence binding site
31-53
Ribosomal Binding Site 143-148
MCS 159-194
FLAG tag 195-218
C-24 Sequencing
MEK 1&2 are also activated by dual-
phosphorylation, which occurs on serines 218 and 222,
in the activation loop of the MEKs. Serine 298 of MEK
2 is phosphorylated by PAK1, which promotes MEK 2
MEK 1&2 are also activated by dual-
phosphorylation, which occurs on serines 218 and 222,
in the activation loop of the MEKs. Serine 298 of MEK
2 is phosphorylated by PAK1, which promotes MEK 2
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
al., International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
, International J. of
Pharmaceutics, 74, 147-156 (1991).
28. Condit, R.C., et al., Virology, 218, 169-180 (1996).
29. Zhang, L. and Gralla, J.D., Genes and
Development 3:1814-1822, Cold Spring
mL.
D. Preparation of lyophilized Cell Lysates (Catalog # 47-218 and 47-216).
A549 Cell Lysate: Camptothecin (# 47-218) is provided as a lyophilized stock of cell
lysate prepared from A549
mL.
D. Preparation of lyophilized Cell Lysates (Catalog # 47-218 and 47-216).
A549 Cell Lysate: Camptothecin (# 47-218) is provided as a lyophilized stock of cell
lysate prepared from A549
phosphatases:
functional inferences from mouse models and human
diseases. FEBS J., 275, 816-30 (2008).
2. Tremblay, M.L., and Giguère, V., Phosphatases at
the heart of FoxO metabolic control.
phosphatases:
functional inferences from mouse models and human
diseases. FEBS J., 275, 816-30 (2008).
2. Tremblay, M.L., and Giguère, V., Phosphatases at
the heart of FoxO metabolic control.
phosphatases:
functional inferences from mouse models and human
diseases. FEBS J., 275, 816-30 (2008).
2. Tremblay, M.L., and Giguère, V., Phosphatases at
the heart of FoxO metabolic control.
phosphatases:
functional inferences from mouse models and human
diseases. FEBS J., 275, 816-30 (2008).
2. Tremblay, M.L., and Giguère, V., Phosphatases at
the heart of FoxO metabolic control.
phosphatases:
functional inferences from mouse models and human
diseases. FEBS J., 275, 816-30 (2008).
2. Tremblay, M.L., and Giguère, V., Phosphatases at
the heart of FoxO metabolic control.