digestion of
1 �g pBR322 DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5
digestion of
1 �g DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM
5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x 1 ml 11 417 991 001
1,4-Dithiothreitol 2 g 10 197 777 001
10 g 10 708 984
5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x 1 ml 11 417 991 001
1,4-Dithiothreitol 2 g 10 197 777 001
10 g 10 708 984
5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x 1 ml 11 417 991 001
1,4-Dithiothreitol 2 g 10 197 777 001
10 g 10 708 984
5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x 1 ml 11 417 991 001
1,4-Dithiothreitol 2 g 10 197 777 001
10 g 10 708 984
5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x 1 ml 11 417 991 001
1,4-Dithiothreitol 2 g 10 197 777 001
10 g 10 708 984
5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x 1 ml 11 417 991 001
1,4-Dithiothreitol 2 g 10 197 777 001
10 g 10 708 984
5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x 1 ml 11 417 991 001
1,4-Dithiothreitol 2 g 10 197 777 001
10 g 10 708 984
5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x 1 ml 11 417 991 001
1,4-Dithiothreitol 2 g 10 197 777 001
10 g 10 708 984
1059–1067 (2016).
7. V. Timm, P. Gruber, M. Wasiliu, H. Lindhofer, and D. Chelius. J
Chrom B 878, 777-784 (2010).
8. K. Sankar, K. H. et. al. MAbs. 10, 1281–1290 (2018).
9. X. M. Lam, J. Y.