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Showing 1-30 of 61 results for "223735" within Papers
Lucia Panzella et al.
Journal of agricultural and food chemistry, 60(36), 8895-8901 (2012-03-20)
An improved purification procedure leading to black sesame ( Sesamum Indicum L.) pigment was developed involving fat removal by treatment of ground black sesame seeds with dichloromethane followed by an optimized hydrolytic protocol with 6 M HCl, at 100 °C
Brijesh Pandey et al.
Protein expression and purification, 79(2), 197-203 (2011-06-29)
A cDNA encoding cinnamyl alcohol dehydrogenase (CAD), catalyzing conversion of cinnamyl aldehydes to corresponding cinnamyl alcohols, was cloned from secondary xylem of Leucaena leucocephala. The cloned cDNA was expressed in Escherichia coli BL21 (DE3) pLysS cells. Temperature and Zn(2+) ion
Sun-Joo Moon et al.
Phytochemistry, 82, 15-21 (2012-08-14)
In this study, dehydrogenative polymers (DHP) were synthesized in vitro through dehydrogenative polymerization using different ratios of coniferyl alcohol (CA) and sinapyl alcohol (SA) (10:0, 8:2, 6:4, 2:8, 0:10), in order to investigate the monolignol coupling mechanism in the presence
Chirantha P Rodrigo et al.
Journal of the American Chemical Society, 133(8), 2632-2641 (2011-02-08)
Single-conformation spectroscopy of the three lignin monomers (hereafter "monolignols") p-coumaryl alcohol (pCoumA), coniferyl alcohol (ConA), and sinapyl alcohol (SinA) has been carried out on the isolated molecules cooled in a supersonic expansion. Laser-induced fluorescence excitation, dispersed fluorescence, resonant two-photon ionization
Heath D Watts et al.
Physical chemistry chemical physics : PCCP, 13(47), 20974-20985 (2011-10-20)
Five potential reaction mechanisms, each leading to the formation of an α-O-4-linked coniferyl alcohol dimer, and one scheme leading to the formation of a recently proposed free-radical coniferyl alcohol trimer were assessed using density functional theory (DFT) calculations. These potential
Yongkun Lv et al.
Bioresource technology, 267, 578-583 (2018-07-29)
Coniferyl alcohol is a valuable chemical. However, the current approaches to obtain coniferyl alcohol are either unefficient or expensive. Penicillium simplicissimum vanillyl alcohol oxidase (PsVAO) can be used to produce coniferyl alcohol. However, PsVAO intrinsically produces harmful byproduct H2O2. Utilizing
Avrajit Chakraborty et al.
Molecular genetics and genomics : MGG, 290(4), 1523-1542 (2015-03-01)
We generated the bast transcriptomes of a deficient lignified phloem fibre mutant and its wild-type jute (Corchorus capsularis) using Illumina paired-end sequencing. A total of 34,163 wild-type and 29,463 mutant unigenes, with average lengths of 1442 and 1136 bp, respectively
Hsi-Chuan Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(52), 21253-21258 (2011-12-14)
The hydroxylation of 4- and 3-ring carbons of cinnamic acid derivatives during monolignol biosynthesis are key steps that determine the structure and properties of lignin. Individual enzymes have been thought to catalyze these reactions. In stem differentiating xylem (SDX) of
Peter Bock et al.
Journal of Raman spectroscopy : JRS, 50(6), 778-792 (2019-07-03)
Anatomical and chemical information can be linked by Raman imaging. Behind every pixel of the image is a Raman spectrum, which contains all the information as a molecular fingerprint. Yet to understand the spectra, the bands have to be assigned
Romina Martínez-Rubio et al.
Physiologia plantarum, 164(1), 45-55 (2018-02-22)
Maize (Zea mays L.) suspension-cultured cells habituated to a cellulose biosynthesis inhibitor 2,6-dichlorobenzonitrile (DCB) have a modified cell wall, in which the reduction in the cellulose content is compensated by a network of highly cross-linked feruloylated arabinoxylans and the deposition
Alif Meem Nurani et al.
Plant & cell physiology, 61(2), 255-264 (2020-01-11)
Stem cells undergo cell division and differentiation to ensure organized tissue development. Because plant cells are immobile, plant stem cells ought to decide their cell fate prior to differentiation, to locate specialized cells in the correct position. In this study
Songkui Cui et al.
The New phytologist, 218(2), 710-723 (2018-03-03)
Parasitic plants in the family Orobanchaceae are destructive weeds of agriculture worldwide. The haustorium, an essential parasitic organ used by these plants to penetrate host tissues, is induced by host-derived phenolic compounds called haustorium-inducing factors (HIFs). The origin of HIFs
Shumei Li et al.
International journal of molecular sciences, 19(8) (2018-08-04)
To investigate the effect of pollination on the fruit quality of 'Dangshan Su' pear, 'Dangshan Su' was fertilized by the pollen of 'Wonhwang' (Pyrus pyrifolia Nakai.) (DW) and 'Jingbaili' (Pyrus ussuriensis Maxim.) (DJ). The analysis of primary metabolites was achieved
Raffaele Guzzon et al.
Journal of food science and technology, 54(3), 810-821 (2017-03-17)
Careful control of spoilage microflora inside wine containers is a key issue during winemaking. To date, attention has been paid to the development of an effective protocol for the eradication of spoilage agents, especially
Richard Sibout et al.
Current biology : CB, 22(13), R533-R535 (2012-07-14)
Lignins are complex aromatic heteropolymers that reinforce the cell walls of terrestrial plants. A new study identifies an ATP-binding cassette ABC transporter that pumps a monolignol lignin precursor across the plasma membrane.
Benjamin Pickel et al.
The FEBS journal, 279(11), 1980-1993 (2012-03-27)
Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol. By an unknown mechanism, they direct the coupling of two phenoxy radicals toward the formation of optically active (+)- or (-)-pinoresinol.
Jiazeng Yang et al.
Metabolic engineering, 59, 44-52 (2020-02-01)
Silymarin extracted from milk thistle seeds, is used for treating hepatic diseases. Silybin and isosilybin are its main components, and synthesized from coupling of taxifolin and coniferyl alcohol. Here, the biosynthetic pathways of taxifolin and coniferyl alcohol were reconstructed in
Xin Wang et al.
The Plant cell, 32(12), 3825-3845 (2020-10-11)
Catechyl lignin (C-lignin) is a linear homopolymer of caffeyl alcohol found in the seed coats of diverse plant species. Its properties make it a natural source of carbon fibers and high-value chemicals, but the mechanism of in planta polymerization of
Anne E Harman-Ware et al.
Biotechnology for biofuels, 10, 281-281 (2017-12-08)
Lignin dehydrogenation polymers (DHPs) are polymers generated from phenolic precursors for the purpose of studying lignin structure and polymerization processes. Here, DHPs were synthesized using a Zutropfverfahren method with horseradish peroxidase and three lignin monomers, sinapyl (S), coumaryl (H), and
Chang-Young Hong et al.
ACS chemical biology, 12(7), 1749-1759 (2017-05-04)
Whole cells of the basidiomycete fungus Phanerochaete chrysosporium (ATCC 20696) were applied to induce the biomodification of lignin in an in vivo system. Our results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin
Lucie Petrásková et al.
Foods (Basel, Switzerland), 9(2) (2020-01-25)
Herbal preparations from Silybummarianum have been used since the fourth century BC in liver disease treatment and against numerous other pathologies. Consumption of silymarin containing drugs and food supplements continues to increase. Precise, fast, reliable, and complex determination of all
Rocío Santiago et al.
Physiologia plantarum, 145(2), 245-259 (2012-01-18)
This study describes a method for determining cinnamyl alcohol dehydrogenase activity in sugarcane stems using reverse phase (RP) high-performance liquid chromatography to elucidate their possible lignin origin. Activity is assayed using the reverse mode, the oxidation of hydroxycinnamyl alcohols into
Kewei Zhang et al.
The Plant cell, 24(7), 3135-3152 (2012-08-02)
Although the practice of protein engineering is industrially fruitful in creating biocatalysts and therapeutic proteins, applications of analogous techniques in the field of plant metabolic engineering are still in their infancy. Lignins are aromatic natural polymers derived from the oxidative
Fengxia Yue et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(51), 16402-16410 (2012-10-31)
Pinoresinol structures, featuring a β-β'-linkage between lignin monomer units, are important in softwood lignins and in dicots and monocots, particularly those that are downregulated in syringyl-specific genes. Although readily detected by NMR spectroscopy, pinoresinol structures largely escaped detection by β-ether-cleaving
Ryoko Baba et al.
Journal of agricultural and food chemistry, 62(33), 8308-8313 (2014-08-05)
The volatile fractions of three famous Chinese green tea cultivar infusions (Longjing, Maofeng, and Biluochun) were prepared by a combination of the adsorptive column method and the SAFE techniques. The aroma extract dilution analysis (AEDA) applied to the volatile fractions
Yuree Lee et al.
Cell, 173(6), 1468-1480 (2018-05-08)
The cell wall, a defining feature of plants, provides a rigid structure critical for bonding cells together. To overcome this physical constraint, plants must process cell wall linkages during growth and development. However, little is known about the mechanism guiding
Motohiro Nishimura et al.
Bioscience, biotechnology, and biochemistry, 75(9), 1770-1777 (2011-09-08)
We purified two isozymes of coniferyl alcohol dehydrogenase (CADH I and II) to homogeneity from cell-free extracts of Streptomyces sp. NL15-2K. The apparent molecular masses of CADH I and II were determined to be 143 kDa and 151 kDa respectively
M P Kolomytseva et al.
International journal of biological macromolecules, 137, 1199-1210 (2019-07-12)
Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (59
Batirtze Prats-Mateu et al.
Scientific reports, 8(1), 11804-11804 (2018-08-09)
Raman microscopy is a powerful imaging technique for biological materials providing information about chemistry in context with microstructure. A 532 nm laser is often used as excitation source, because high spatial resolution and signal intensity can be achieved. The latter can
Le Thanh Mai Pham et al.
Protein expression and purification, 80(2), 268-273 (2011-08-23)
Cationic cell wall peroxidase (CWPO_C) from poplar tree (Populus alba L) was heterologously expressed in Escherichia coli as an inclusion body. The insoluble inclusion body was solubilized and reactivated via a refolding procedure. The condition for this procedure was optimized
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