assayed by flow cytometric analysis, using the
antibody at working concentration to stain 1 x 106 to
2 x 106 cells/0.1ml/test, fluorescence intensity is
observed similar to that obtained with saturating
assayed by flow cytometric analysis, using the
antibody at working concentration to stain 1 x 106 to
2 x 106 cells/0.1ml/test, fluorescence intensity is
observed similar to that obtained with saturating
2nd edition, Gunstone, F. D.,
et al., Chapman and Hall (London, UK), dictionary
section p 106.
2. Fox, J., Fatty acids' spontaneous oxidation clarified.
Chemical and Engineering News, pages 18
with type 2
diabetes mellitus. Nature Genetics, 26, 163-175
(2000).
6. Permutt, M.A., et al., Calpain 10: the first positional
cloning of a gene for type 2 diabetes? J. Clin.
Invest., 106, 819-821
with type 2
diabetes mellitus. Nature Genetics, 26, 163-175
(2000).
6. Permutt, M.A., et al., Calpain 10: the first positional
cloning of a gene for type 2 diabetes? J. Clin.
Invest., 106, 819-821
with type 2
diabetes mellitus. Nature Genetics, 26, 163-175
(2000).
6. Permutt, M.A., et al., Calpain 10: the first positional
cloning of a gene for type 2 diabetes? J. Clin.
Invest., 106, 819-821
with type 2
diabetes mellitus. Nature Genetics, 26, 163-175
(2000).
6. Permutt, M.A., et al., Calpain 10: the first positional
cloning of a gene for type 2 diabetes? J. Clin.
Invest., 106, 819-821
2nd edition, Gunstone, F. D.,
et al., Chapman and Hall (London, UK), dictionary
section p 106.
2. Fox, J., Fatty acids' spontaneous oxidation clarified.
Chemical and Engineering News, pages 18
2nd edition, Gunstone, F. D.,
et al., Chapman and Hall (London, UK), dictionary
section p 106.
2. Fox, J., Fatty acids' spontaneous oxidation clarified.
Chemical and Engineering News, pages 18
1. Collect 2-3 x 107 Jurkat cells by centrifugation and
resuspend the cells in Jurkat growth medium at
approximately 2-3 x 106 cell per ml in
approximately 10 ml
2. Dispense 2 ml of cell
immunoprecipitation of
proteins.
Cat. No. 11 719 416 001 Protein G Agarose
2 ml
Cat. No. 11 719 408 001 Protein A Agarose
2 ml
Cat. No. 11 243 233 001 Protein G Agarose
5 ml
immunoprecipitation of
proteins.
Cat. No. 11 719 416 001 Protein G Agarose
2 ml
Cat. No. 11 719 408 001 Protein A Agarose
2 ml
Cat. No. 11 243 233 001 Protein G Agarose
5 ml
Passage every
2-3 days by rinsing with phosphate buffered saline before harvesting with
Trypsin/EDTA and seeding into new flasks using a seeding density of 0.5-1×106
cells per T75 cm2 or 1-2×106 cells per
Passage every
2-3 days by rinsing with phosphate buffered saline before harvesting with
Trypsin/EDTA and seeding into new flasks using a seeding density of 0.5-1×106
cells per T75 cm2 or 1-2×106 cells per
or PBMCs. For optimal throughput, final cell concentrations should be between 2 x 104 and 1 x
105 cells/well (or 2 x 106 to 1 x 107 cells/mL). EMD-Millipore recommends using the ViaCount™
reagent to
or PBMCs. For optimal throughput, final cell concentrations should be between 2 x 104 and 1 x
105 cells/well (or 2 x 106 to 1 x 107 cells/mL). EMD-Millipore recommends using the ViaCount™
reagent to
or PBMCs. For optimal throughput, final cell concentrations should be between 2 x 104 and 1 x
105 cells/well (or 2 x 106 to 1 x 107 cells/mL). EMD-Millipore recommends using the ViaCount™
reagent to
inhibition causing senescence and should thus
be passaged every 2-3 days using a seeding density of 0.5-1×106 cells per T75
cm2 or 1-2×106 cells per T175 cm2 flask. Pre-washing with phosphate buffered
cultures. Optimal Staining is obtained for cell concentrations between 2 x 105 and
2.5 x 104 cells/well (or 2 x 107 to 2.5x 106 cells/mL). Millipore recommends using the ViaCount™
reagent to obtain accurate
11 647 652 001
Protein A-agarose 2 ml 11 719 408 001
Protein A-agarose 5 ml 11 134 515 001
Protein G-agarose 2 ml 11 719 416 001
Protein G-agarose 5 ml 11 243 233 001
BM Chemiluminescence Blotting Kit
11 647 652 001
Protein A-agarose 2 ml 11 719 408 001
Protein A-agarose 5 ml 11 134 515 001
Protein G-agarose 2 ml 11 719 416 001
Protein G-agarose 5 ml 11 243 233 001
BM Chemiluminescence Blotting Kit