Synthetic peptide corresponding to amino acids 242-258 of rat or human P2Y1 (SwissProt
accession numbers P49651, P47900).
APPLICATIONS: Western blot: 1:200-1:400 using ECL on rat brain membranes or human
Synthetic peptide corresponding to amino acids 242-258 of rat or human
P2Y1 (SwissProt accession numbers P49651, P47900).
APPLICATIONS: Western blot: 1:200-1:400 using ECL on rat brain membranes or human
or better) can be added (1:1) for additional stability.
REFERENCES: Zhou XF and Rush RA, Neuroscience (1996) 74:945-953.
Zhou XF et al., Exp, Neurol (1998) 149:237-242.
RELATED REFERENCES: Acheson
Dyn., 235, 1152-1166 (2006).
6. Sanchez-Camacho, C., et al., Brain Res. Rev., 49,
242-252 (2004).
PHC 07/11-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants
Dev. Dyn., 235, 1152-1166 (2006). 6. Sanchez-Camacho, C., et al., Brain Res. Rev., 49,
242-252 (2004).
PHC 07/11-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants
Preparation
1. Before opening the vial, spin down the solid to the bottom by a microcentrifuge or by a desktop
centrifuge.
2. Warm the vial to the room temperature and add 242 µL of
on IonWorksTM HT. Representative
success rates per 384 well plate were as follows. 63% of cells (n=242 cells)
sealed and of these, 81% (n=198 cells) had currents above 200 pA. The mean
peak current of
Corporation, 28820 Single Oak Drive, Temecula, CA 92590 USA 1-800-437-7500
Technical Support T 1-800-MILLIPORE (1-800-645-4576) F 1-800-437-7502
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Corporation, 28820 Single Oak Drive, Temecula, CA 92590 USA 1-800-437-7500
Technical Support T 1-800-MILLIPORE (1-800-645-4576) F 1-800-437-7502
www.millipore.com
This page
Corporation, 28820 Single Oak Drive, Temecula, CA 92590 USA 1-800-437-7500
Technical Support T 1-800-MILLIPORE (1-800-645-4576) F 1-800-437-7502
www.millipore.com
Corporation, 28820 Single Oak Drive, Temecula, CA 92590 USA 1-800-437-7500
Technical Support T 1-800-MILLIPORE (1-800-645-4576) F 1-800-437-7502
www.millipore.com
427 MAP Kinase Phosphatase-1 (MKP-1) M3787 1843 DUSP1 P NP_004408.1 y n/d n/d
239 E2F1 E9026 1869 E2F1 P NP_005216.1 y n/d n/d
241 E2F1 E8901 1869 E2F1 M NP_005216.1 y y y
242 E2F2 E8776 1870 E2F2 M NP_
427 MAP Kinase Phosphatase-1 (MKP-1) M3787 1843 DUSP1 P NP_004408.1 y n/d n/d
239 E2F1 E9026 1869 E2F1 P NP_005216.1 y n/d n/d
241 E2F1 E8901 1869 E2F1 M NP_005216.1 y y y
242 E2F2 E8776 1870 E2F2 M NP_
427 MAP Kinase Phosphatase-1 (MKP-1) M3787 1843 DUSP1 P NP_004408.1 y n/d n/d
239 E2F1 E9026 1869 E2F1 P NP_005216.1 y n/d n/d
241 E2F1 E8901 1869 E2F1 M NP_005216.1 y y y
242 E2F2 E8776 1870 E2F2 M NP_
y
436 MAP2 M9942 M y y y
437 MBD1 M6569 P y n/d n/d
438 MBD2a M7568 P y y n/d
439 MBD2a,b M7318 P y y n/d
440 MBD4 M9817 P n/d y n/d
441 MBDin/XAB1 M1944 P y n/d n/d
442 MBNL 1 M3320
Anti Cy3+Cy5
241 E2F1 E8901 399489, 13555, 1869 E2F1,E2f1 XP_230765.4,NP_031917.1,NP_005216.1 M y y y
242 E2F2 E8776 1870 E2F2 NP_004082.1 M y n/d n/d
243 E2F3 E8651 1871 E2F3 NP_001940.1 M y n/d n/d
244 E2F4
Cy5 C0992
241 E2F1 E8901 399489, 13555, 1869 E2F1,E2f1 XP_230765.4,NP_031917.1,NP_005216.1 M y y y
242 E2F2 E8776 1870 E2F2 NP_004082.1 M y n/d n/d
243 E2F3 E8651 1871 E2F3 NP_001940.1 M y n/d n/d
244 E2F4