Eagle’s Medium (DMEM)
with 10% fetal bovine serum (e.g., Product No.
F 2442).
13. Repeat step 10 to (undissociated) minced
tissues.
14. Mix slowly (e.g., 40 rpm) for 10 minutes at 37 ºC.
Do not
Anti-Dopamine Transporter
Developed in Rabbit
Whole Antiserum
Product Number D 2442
Product Description
Anti-Dopamine Transporter (DAT) is developed in rabbit
using as immunogen
Liver, Pooled (S2442) - Datasheet
S9 from Liver, Pooled
from human
Product Number S 2442
Storage Temperature −70 °C
Product Description
This product is a buffered solution containing
chicken fibroblasts) in DMEM
medium (Sigma Product No. D 5546) with 10% fetal
calf serum (Sigma Product F 2442) .
3. 10 mM phosphate buffered saline (PBS) pH 7.2-7.4
(Sigma Product No. P 4417
human or chicken fibroblasts) in DMEM
medium (Product No. D 5546) with 10% fetal calf
serum (Product No. F 2442)
3. 10 mM phosphate buffered saline (PBS) pH 7.2 to
7.4 (Product No. P 4417)
Modified Eagle Medium (DMEM)
(Product No. D 5671).
4. Fetal Bovine Serum (FBS) (Product No. F 2442).
5. Phosphate Buffered Saline (PBS) (Product No.
P 3813).
Procedure
Note: Due to the rapid
Dulbecco’s Modified Eagle’s
Medium (DMEM; Product Code: D 6171) containing
10% Fetal Bovine Serum (FBS; Product Code: F 2442) in
100-mm tissue culture plates (Product Code: C 6546).
When the cells were
Cells were cultured in RPMI-1640 medium (Product Code
R 5886) supplemented with 10% fetal bovine serum
(Product Code F 2442), 2 mM L-glutamine (Product Code
G 7513), 0.1% b-mercaptoethanol (Product Code
for the E1 region. Per.C6® cells were cultured
as attached cells in T-flasks in DMEM + 10% FBS (Product
Code F 2442). Agitated small-scale suspension cultures
were grown in 125 ml-1 L spinner flasks in
Acad. Sci. USA, 98, 13049-13054 (2001).
19. Honda, A., et al., Proc. Natl. Acad. Sci. USA, 98, 2437-2442 (2001).
20. Zaccolo, M., et al., Curr. Opin. Cell Biol., 14, 160-166 (2002).
21. Xie, F. and Conti