intermolecular
charge transfer fluorophore and its in vitro and in
vivo application, J. Amer. Chem. Soc., 131, 1460-
1468, (2009).
PD,MS,VNC,MAM 05/10-1
N
N
N
N
N
ON
NO2
N
2
3rd ed., Vol. III, Boyer,
P.D., Academic Press (New York, NY: 1971), pp.
250-275.
4. Shaw, E., et al., Biochemistry, 4(10), 2219-2224
(1965).
5. Buck, F.F., et al., Arch. Biochem. Biophys.
241 amino acids in length, with three peptide chains:1
• A chain: 13 residues
• B chain: 131 residues
• C chain: 97 residues
α-Chymotrypsin from bovine pancreas selectively
catalyzes
, in The Enzymes, 3rd ed., Vol. III, Boyer,
P.D., Academic Press (New York, NY: 1971),
pp. 250-275.
4. Shaw, E. et al., Evidence for an active center
histidine in trypsin through use of a specific
, in The Enzymes, 3rd ed., Vol. III, Boyer,
P.D., Academic Press (New York, NY: 1971),
pp. 250-275.
4. Shaw, E. et al., Evidence for an active center
histidine in trypsin through use of a specific
conducted on various resins.
Abbreviations: DFPE: 2-(3,5-Dimethoxy-4-formylphenoxy)ethyl;
FMPB: 4-(Formyl-3-methoxy-phenoxy)butyryl.
Method 1
250 mg of each resin were suspended in either NMP or DCE (4.75 ml
in The Enzymes, 3rd ed., Vol. III, Boyer, P.
D., Academic Press (New York, NY: 1971),
pp. 250-275.
4. Burdon, R.H. et al., in Laboratory Techniques in
Biochemistry and Molecular Biology, Vol. 9
in The Enzymes, 3rd ed., Vol. III, Boyer, P.
D., Academic Press (New York, NY: 1971),
pp. 250-275.
4. Burdon, R.H. et al., in Laboratory Techniques in
Biochemistry and Molecular Biology, Vol. 9
in The Enzymes, 3rd ed., Vol. III, Boyer, P.
D., Academic Press (New York, NY: 1971),
pp. 250-275.
4. Burdon, R.H. et al., in Laboratory Techniques in
Biochemistry and Molecular Biology, Vol. 9
PVC
Operating Temperature
Volume Indicator Ruler –15 to 55 °C (5 to 131 °F)
Crimped Tube
Protector Caps
–20 to 60 °C (–4 to 140 °F)
Dimensions Thickness (µm) Volume (mL) Length x Width (mm)
extend the
production phase in fed-batch mode. This single feed is highly
concentrated, at more than 131 g/L, allowing for a reduction
in volume of feed added to the medium thereby increasing
product yield
Connected 60 psig (4.1 bar)
Sterilization Capability
Gamma up to 45 kGy
Autoclave up to 131 °C, 30 minutes, 3 cycles
Temperature Ratings
Storage 0-40 °C (32-104 ºF) environmental temperature
Buffered Peptone Water
• A 3 liter bag of concentrate produces
30 liters of media, enough for 131
samples of 25 g
• A 10 liter bag of concentrate
produces 100 liters of media,
enough for
Buffered Peptone Water
• A 3 liter bag of concentrate produces
30 liters of media, enough for 131
samples of 25 g
• A 10 liter bag of concentrate
produces 100 liters of media,
enough for
)
Operating Temperature
Volume Indicator Ruler –15 to 55 °C (5 to 131 °F)
Crimped Tube Protector Caps –20 to 60 °C (–4 to 140 °F)
First actuation locking tag
ed., Vol. III, Boyer,
P.D. (ed.). Academic Press (New York, NY),
pp. 250-275 (1971).
4. Shaw, E. et al., Biochemistry, 4(10), 2219-2224
(1965).
5. Buck, F.F. et al., Arch. Biochem. Biophys
Brown, N. L. (1985) J. Gen. Microbiol. 131,
951–958.
2 Kessler, C. & Höltke, H.-J. (1986) Gene 47, 1–153.
3 van Heuverswyn, H. & Fiers, W. (1980) Gene 9, 195-203.
4 Rebase The Restriction Enzyme Database
cell-autonomously required to prevent
apoptosis in mesencephalic dopaminergic neurons. Development 131(13) : 3229-3236.
Baker, SA et al. (2004). Dopaminergic nigrostriatal projections regulate neural