PCDH10 Hu-Cy5
SmartFlare™
RNA Detection Probe
Cat. # SF-2610
pack size: 50µL (250 rxns)
Store at 2-8°C,
after reconstitution store at 23-27°C
DO NOT FREEZE
al., Molecular cloning of the cDNA
encoding human deoxyribonuclease II. J. Biol.
Chem., 273, 2610-2616 (1998).
2. Krieser, R.J., and Eastman, A.,. The cloning and
expression of human deoxyribonuclease
MONOCLONAL ANTI-p14ARF
CLONE DCS-240
Purified Mouse Immunoglobulin
Product Number P 2610
Product Description
Monoclonal Anti-p14ARF (mouse IgG1 isotype) is derived
from the DCS-240 hybridoma produced
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
10.
8. Enzyme Solution F (green screw cap), dilute a suitable amount 1:100 with
washing solution E before usage
0.140 mL
9. Substrate Solution G b) (green cap), ready to use 10 mL
and sample, 10 min)
5. Immobilisation
(Binding of the „sandwiches“
to an affinity plate, 10 min)
6. Enzym coupling
(Coupling of an enzyme
to the „sandwiches“, 10 min)
8. Encymatic reaction
thermoshaker
8. Add 10 µL of the supernatant from step 6 to each well (row A1–H1 is reserved for the respective
standards); cover the microwell plate with a lid and incubate for 10 min at 50°C and