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Showing 1-30 of 47 results for "277827" within Papers

Statin-mediated inhibition of cholesterol synthesis induces cytoprotective autophagy in human leukemic cells.

Urosh Vilimanovich et al.

European journal of pharmacology, 765, 415-428 (2015-09-12)

Statins exhibit anti-leukemic properties due to suppression of the mevalonate pathway by the inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase, and subsequent depletion of cholesterol, farnesylpyrophosphate, and geranylgeranylpyrophosphate. We investigated the role of autophagy, a controlled intracellular self-digestion, in the anti-leukemic

Evaluation of gas-liquid chromatography (GLC) for rapid detection of Clostridium difficile in fecal specimens.

C Nonhoff et al.

Acta clinica Belgica, 50(2), 76-80 (1995-01-01)

Clostridium difficile intestinal infection is a major nosocomial hazard in patients receiving antimicrobial therapy. Rationale for rapid diagnosis include lifesaving antimicrobial therapy in patients with severe colitis and early isolation measures for transmission control. We have therefore analysed the sensitivity

Metabolic response to nonglucidic nutrient secretagogues and enzymatic activities in pancreatic islets of adult rats after neonatal streptozotocin administration.

A Sener et al.

Biochemical medicine and metabolic biology, 49(2), 182-199 (1993-04-01)

In islets from adult rats injected with streptozotocin during the neonatal period, both a nonmetabolized analog of L-leucine and 3-phenylpyruvate augmented 14CO2 output from islets either prelabeled with L-[U-14C]glutamine or exposed to D-[2-14C]glucose and D-[6-14C]glucose, in a manner qualitatively comparable

Development and performance evaluation of a novel dynamic headspace vacuum transfer "In Trap" extraction method for volatile compounds and comparison with headspace solid-phase microextraction and headspace in-tube extraction.

Pascal Fuchsmann et al.

Journal of chromatography. A, 1601, 60-70 (2019-06-11)

Headspace in-tube extraction (HS-ITEX) and solid phase microextraction (HS-SPME) sampling, followed by gas chromatography-mass spectrometry (GC-MS), are widely used to analyze volatile compounds in various food matrices. While the extraction efficiency of volatile compounds from foodstuffs is crucial for obtaining

An early steroidogenic defect in hormone-induced Leydig cell desensitization.

M A Hattori et al.

Journal of steroid biochemistry, 21(3), 265-277 (1984-09-01)

To define the nature of the lesion of the early steroidogenic pathway (prior to pregnenolone formation) in gonadotropin-induced desensitization of rat testicular Leydig cells, we evaluated cholesterol side-chain cleavage activity in isolated mitochondria by measurement of pregnenolone synthesis and [14C]isocaproic

Spatial Compartmentalization of the Microbiome between the Lumen and Crypts Is Lost in the Murine Cecum following the Process of Surgery, Including Overnight Fasting and Exposure to Antibiotics.

Alexander Zaborin et al.

mSystems, 5(3) (2020-06-11)

The cecum is a unique region in the mammalian intestinal tract in which the microbiome is localized to two compartments, the lumen and the crypts. The microbiome within crypts is particularly important as it is in direct contact with lining

Structural features of soluble cereal arabinoxylan fibers associated with a slow rate of in vitro fermentation by human fecal microbiota.

Pinthip Rumpagaporn et al.

Carbohydrate polymers, 130, 191-197 (2015-06-17)

Most soluble dietary fibers ferment rapidly in the proximal colon, potentially causing discomfort and poor tolerability. Alkali-extracted arabinoxylan isolates from corn, wheat, rice and sorghum brans were prepared, through hydrolysis (except sorghum) and ethanol fractionation, to have a broad range

Liver breath tests non-invasively predict higher stages of non-alcoholic steatohepatitis.

Piero Portincasa et al.

Clinical science (London, England : 1979), 111(2), 135-143 (2006-04-11)

Effectively assessing subtle hepatic metabolic functions by novel non-invasive tests might be of clinical utility in scoring NAFLD (non-alcoholic fatty liver disease) and in identifying altered metabolic pathways. The present study was conducted on 39 (20 lean and 19 obese)

Gut metabolites and bacterial community networks during a pilot intervention study with flaxseeds in healthy adult men.

Ilias Lagkouvardos et al.

Molecular nutrition & food research, 59(8), 1614-1628 (2015-05-20)

Flaxseeds contain the phytoestrogens lignans that must be activated to enterolignans by intestinal bacteria. We investigated the impact of flaxseeds on fecal bacterial communities and their associations with fecal and blood metabolites. Nine healthy male adult subjects ingested 0.3 g/kg/day

Cholesterol side-chain cleavage activity in the human umbilical cord in vitro.

R Gunasegaram et al.

Journal of perinatal medicine, 18(6), 495-499 (1990-01-01)

With a view to establish whether cells of the human umbilical cord possess cholesterol side-chain cleavage activity, homogenates of term umbilical cord obtained following spontaneous vaginal delivery from uncomplicated pregnancies (n = 6; 38-40 weeks gestation) were incubated with 26-14C

Fecal microbiota responses to rice RS3 are specific to amylose molecular structure.

Fangting Gu et al.

Carbohydrate polymers, 243, 116475-116475 (2020-06-14)

Resistant starch type 3 (RS3) benefits colon health, but the molecular structural reasons for this effect are unclear. Five rice starches with varied amylose content (19.1 %-40.6 %) were used to investigate their effect on gut microbiota. Size-exclusion chromatography and

Anaesthetic action of esters and ketones: evidence for an interaction with the sodium channel protein in squid axons.

J R Elliott et al.

The Journal of physiology, 354, 407-418 (1984-09-01)

The effects of methyl butyl ketone, methyl heptyl ketone and methyl pentanoate on the sodium current of the squid giant axon have been examined. The peak inward current in intact axons was reduced reversibly by each substance. Sodium currents were

Skeletal muscle myosin heavy chain synthesis in type 1 diabetes.

M R Charlton et al.

Diabetes, 46(8), 1336-1340 (1997-08-01)

Although insulin's anticatabolic effect on protein metabolism in type 1 diabetes has been clearly shown to be related to the inhibition of protein breakdown, insulin's effect on muscle protein synthesis remains controversial. Cross-limb studies and measurements of synthesis rates of

Latex particle agglutination for detecting and identifying Clostridium difficile.

R A Bowman et al.

Journal of clinical pathology, 39(2), 212-214 (1986-02-01)

A total of 329 selective enrichment broth cultures were tested for detection of Clostridium difficile by latex particle agglutination (LPA), gas-liquid chromatography, and bacterial culture. Of 53 broths positive by LPA, 36 were positive by gas-liquid chromatography, and 42 were

Evaluation of gas-liquid chromatography for the rapid diagnosis of Clostridium difficile associated disease.

P Gianfrilli et al.

Journal of clinical pathology, 38(6), 690-693 (1985-06-01)

Direct gas-liquid chromatography of faecal specimens with isocaproic acid as a marker was used for the rapid diagnosis of Clostridium difficile associated diarrhoeal diseases. Ninety stools were examined and results were compared with conventional culture on selective medium and cytotoxin

Early infancy microbial and metabolic alterations affect risk of childhood asthma.

Marie-Claire Arrieta et al.

Science translational medicine, 7(307), 307ra152-307ra152 (2015-10-02)

Asthma is the most prevalent pediatric chronic disease and affects more than 300 million people worldwide. Recent evidence in mice has identified a "critical window" early in life where gut microbial changes (dysbiosis) are most influential in experimental asthma. However

Diet and specific microbial exposure trigger features of environmental enteropathy in a novel murine model.

Eric M Brown et al.

Nature communications, 6, 7806-7806 (2015-08-05)

Environmental enteropathy (EE) is a subclinical chronic inflammatory disease of the small intestine and has a profound impact on the persistence of childhood malnutrition worldwide. However, the aetiology of the disease remains unknown and no animal model exists to date

Subcellular location of enzymes involved in leucine dissimilation in Clostridium bifermentans.

M L Britz et al.

Canadian journal of microbiology, 29(4), 441-447 (1983-04-01)

Conversion of leucine to isovaleric (iV) and isocaproic (iC) acids by cell-free extracts of Clostridium bifermentans was demonstrated using two lysis procedures. Sonication resulted in an extract which had the enzymes to convert leucine to alpha-ketoisocaproic acid (alpha-kiC) and thence

Enzymes enhance degradation of the fiber-starch-protein matrix of distillers dried grains with solubles as revealed by a porcine in vitro fermentation model and microscopy.

R Jha et al.

Journal of animal science, 93(3), 1039-1051 (2015-05-29)

Effects of treating corn and wheat distillers dried grains with solubles (DDGS) with a multicarbohydrase alone or in combination with a protease on porcine in vitro fermentation characteristics and the matrix structure of the DGGS before and after the fermentation

Adaptation of the cecal bacterial microbiome of growing pigs in response to resistant starch type 4.

Barbara U Metzler-Zebeli et al.

Applied and environmental microbiology, 81(24), 8489-8499 (2015-10-04)

Resistant starch (RS) exacerbates health benefits on the host via modulation of the gut bacterial community. By far, these effects have been less well explored for RS of type 4. This study aimed at gaining a community-wide insight into the

Multiphasic control of hepatic protein degradation by regulatory amino acids. General features and hormonal modulation.

G E Mortimore et al.

The Journal of biological chemistry, 262(34), 16322-16327 (1987-12-05)

Previous studies with livers from fed rats perfused in the single-pass mode have shown that regulatory amino acids (Leu, Tyr, Gln, Pro, Met, His, and Trp) as a group as well as leucine alone inhibit deprivation-induced protein degradation optimally at

Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement.

Chizuka Tokuda et al.

Journal of bacteriology, 185(16), 5023-5026 (2003-08-05)

The single amino acid replacement of Tyr52 with Leu drastically increased the activity of Lactobacillus pentosus NAD-dependent D-lactate dehydrogenase toward larger aliphatic or aromatic 2-ketoacid substrates by 3 or 4 orders of magnitude and decreased the activity toward pyruvate by

Separation and conductimetric detection of C1-C7 aliphatic monocarboxylic acids and C1-C7 aliphatic monoamines on unfunctionized polymethacrylate resin columns.

Kazutoku Ohta et al.

Journal of chromatography. A, 1039(1-2), 171-177 (2004-07-15)

The application of unfunctionized polymethacrylate resin (TSKgel G3000PWXL) as a stationary phase in liquid chromatography with conductimetric detection for C1-C7 aliphatic monocarboxylic acids (formic acid, acetic acid, propionic acid, butyric acid, isovaleric acid, valeric acid, 3,3-dimethylbutyric acid, 4-methylvaleric acid, hexanoic

Biosynthesis of novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate by Chromobacterium sp. USM2.

S C Ling et al.

Journal of applied microbiology, 111(3), 559-571 (2011-06-22)

Polyhydroxyalkanoate (PHA) with enhanced physicochemical properties will be ideal for a wide range of practical applications. The incorporation of 3-hydroxy-4-methylvalerate (3H4MV) into the polymer backbone is known to improve the overall properties of the resulting polymer. However, the most suitable

In Vitro Fecal Fermentation of High Pressure-Treated Fruit Peels Used as Dietary Fiber Sources.

Viridiana Tejada-Ortigoza et al.

Molecules (Basel, Switzerland), 24(4) (2019-02-17)

Fruit by-products are being investigated as non-conventional alternative sources of dietary fiber (DF). High hydrostatic pressure (HHP) treatments have been used to modify DF content as well as its technological and physiological functionality. Orange, mango and prickly pear peels untreated

In vitro fecal fermentation of propionylated high-amylose maize starch and its impact on gut microbiota.

Zhuqing Xie et al.

Carbohydrate polymers, 223, 115069-115069 (2019-08-21)

Resistant starch (RS) acylated with propionate groups is of particular interest in terms of their capacity to deliver specific short-chain fatty acids (SCFAs) to the colon with health benefits. In the present study, we fabricated propionylated high-amylose maize starches with

Cytochrome P-450 cholesterol side-chain cleavage expression in human spermatozoa.

R Gunasegaram et al.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme, 30(1), 58-60 (1998-03-21)

Isocaproic acid, a metabolite of 20 alpha-hydroxycholesterol.

K SHIMIZU et al.

The Journal of biological chemistry, 235, PC25-PC25 (1960-06-01)

Detection of Clostridium difficile in faeces by direct gas liquid chromatography.

P N Levett

Journal of clinical pathology, 37(2), 117-119 (1984-02-01)

Stool specimens examined for the presence of Clostridium difficile and its cytotoxin were screened by gas liquid chromatography for the presence of volatile fatty acids and p-cresol. Twenty seven of 110 (25%) stools yielded C difficile or cytotoxin; iso-valeric acid

Olfactometry Profiles and Quantitation of Volatile Sulfur Compounds of Swiss Tilsit Cheeses.

Pascal Fuchsmann et al.

Journal of agricultural and food chemistry, 63(34), 7511-7521 (2015-08-01)

To establish the odor profiles of three differently fabricated commercial Swiss Tilsit cheeses, analyses were conducted using headspace solid-phase microextraction gas chromatography-mass spectrometry/pulsed flame photometric detection and gas chromatography-olfactometry to identify and quantitate volatile compounds. In addition, odor quality and

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