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Scepter™ 3.0 Cell Counter – How it Works

Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Authenticated Breast Cancer Cell Lines for Cancer Research

Choose from more than 30 breast cancer cell lines authenticated by ECACC, classified by hormone receptor-positive, HER2-positive, and triple-negative cancers, as well as by known mutations such as BRCA2.

Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber

Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt

Assay of Betamethasone Sodium Phosphate by HPTLC

Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

RNase, DNase-free Protocol

0.1 mU RNase, DNase-free degrades 1 μg RNA in 30 min at + 37 °C in a reaction volume of 50 μL PCR grade water. The protein concentration of RNase, DNase-free is 0.5 μg/μL.

3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

New Solar Panels at Merck’s Wisconsin, USA Site Add Renewable Energy to Power Grid

The Life Science business of Merck, a leading science and technology company, adds 2.25 megawatts to the grid with 7,000 solar panels at its Sheboygan, Wisconsin, USA site, which are now fully installed. The project is part of a 30-year

Meeting on the Mesa

Connect with us during Meeting on the Mesa to learn more about our viral vector CDMO solutions for cell and gene therapies. Backed by 30 years of experience and superior regulatory compliance, we have supported the development and manufacturing of

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