Cloning and characterization of
a member of the MST subfamily of Ste20-like
kinases. Gene, 167, 303-306 (1995).
2. Lee, K.K. et al., MST, a physiological caspase
substrate, highly sensitizes apoptosis
Cloning and characterization of
a member of the MST subfamily of Ste20-like
kinases. Gene, 167, 303-306 (1995).
2. Lee, K.K. et al., MST, a physiological caspase
substrate, highly sensitizes apoptosis
reactions in a final volume of 50 μl 12 140 306 001
Pwo SuperYield DNA
Polymerase
100 U, 5 U/μl, 40 reactions in a final volume of 50 μl 04 340 868 001
2 x 250 U, 5 U/μl, 200 reactions
K.O. et al., J. Biol. Chem., 271, 33325-33334
(1996).
3. Morelle, W. et al., Carbohydr. Res., 306, 435-443
(1998).
GlycoProfile is a trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich
human histone H2A.X gene to 11q23.2-q23.3
by fluorescence in situ hybridization. Hum. Genet.
94: 303-306, (1994).
3. Bassing, C. H., et al., Histone H2AX: a dosage-
dependent suppressor of oncogenic
to 5 kb
Yes 4
FastStart High
Fidelity PCR
System (Hot start)
125 U
2× 250 U
10× 250 U
03 553 426 001
03 553 400 001
03 553 361 001
No 3 GC-RICH PCR System 100 U
High Fidelity PCR System 5 U/μl, dNTPack
sigma-aldrich.com 7
Step Temperature [°C] Time Number of Cycles
Pre-Incubation 94 2 min 1
Denaturation
Annealing
Elongation
94
55 – 68
68
10 – 30
1 U/μl 11 775 375 001
GC-RICH PCR System 100 U, 50 reactions in a final volume of 50 μl 12 140 306 001
High Pure PCR Product
Purification Kit
1 kit, up to 50 purifications 11 732 668