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Showing 1-30 of 114 results for "408220" within Papers
Da Un Jeong et al.
Biomedical materials (Bristol, England) (2020-11-06)
Excessive and prolonged neuroinflammation leads to neuronal cell death and limits functional recovery after traumatic brain injury (TBI). Dexamethasone (DX) is a steroidal anti-inflammatory agent that is known to attenuate early expression of pro-inflammatory cytokines associated with activated microglia/macrophages. In
Baoju Wang et al.
Journal of biophotonics, 13(5), e2419-e2419 (2020-01-31)
Either modulated illumination or temporal fluctuation analysis can assist super-resolution techniques in overcoming the diffraction limit of conventional optical microscopy. As they are not contradictory to each other, an effective combination of spatial and temporal super-resolution mechanisms would further improve
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Weng Man Chong et al.
eLife, 9 (2020-04-04)
Subdistal appendages (sDAPs) are centriolar elements that are observed proximal to the distal appendages (DAPs) in vertebrates. Despite the obvious presence of sDAPs, structural and functional understanding of them remains elusive. Here, by combining super-resolved localization analysis and CRISPR-Cas9 genetic
Courtney A Burger et al.
eLife, 9 (2020-05-08)
Structural changes in pre and postsynaptic neurons that accompany synapse formation often temporally and spatially overlap. Thus, it has been difficult to resolve which processes drive patterned connectivity. To overcome this, we use the laminated outer murine retina. We identify
Daniel Josef Bell et al.
Soft matter, 17(3), 592-599 (2020-11-18)
Stimuli-responsive polymers are capable of reacting to an external trigger. We report self-regulated, enzymatically active, and pH-responsive hydrogels that show dynamic behavior without an external trigger. This is enabled by a feedback loop between the enzymatic conversion of glucose into
Davide Gambarotto et al.
Nature methods, 16(1), 71-74 (2018-12-19)
Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for
Ye Li et al.
Frontiers in neural circuits, 15, 732183-732183 (2021-11-09)
Identifying the cellular origins and mapping the dendritic and axonal arbors of neurons have been century old quests to understand the heterogeneity among these brain cells. Current Brainbow based transgenic animals take the advantage of multispectral labeling to differentiate neighboring
Dora Mahecic et al.
Nature methods, 17(7), 726-733 (2020-06-24)
Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for field-independent imaging (mfFIFI). By integrating mfFIFI into an
Jervis Vermal Thevathasan et al.
Nature methods, 16(10), 1045-1053 (2019-09-29)
Quantitative fluorescence and superresolution microscopy are often limited by insufficient data quality or artifacts. In this context, it is essential to have biologically relevant control samples to benchmark and optimize the quality of microscopes, labels and imaging conditions. Here, we
Israr Ahmad et al.
Colloids and surfaces. B, Biointerfaces, 159, 540-545 (2017-08-29)
Inhibiting amyloid β (Aβ) aggregation has drawn much attention because it is one of the main reasons for the cause of Alzheimer's disease (AD). Here we have synthesized a nanocomposite of graphene oxide-iron oxide (GOIO) and demonstrated its ability of
Tyler Quoc-Thai Do et al.
iScience, 23(11), 101664-101664 (2020-11-03)
Stress adaptation is exploited by cancer cells to survive and proliferate under adverse conditions. Survival pathways induced by stress are thus highly promising therapeutic targets. One key pathway involves formation of cytoplasmic stress granules, which regulate the location, stability, and
Emmanuelle Steib et al.
eLife, 9 (2020-09-19)
Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together by an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms
Peter Gorilak et al.
Open biology, 11(9), 210131-210131 (2021-09-02)
Expansion microscopy (ExM) has become a powerful super-resolution method in cell biology. It is a simple, yet robust approach, which does not require any instrumentation or reagents beyond those present in a standard microscopy facility. In this study, we used
Nora Trinks et al.
Communications biology, 4(1), 1151-1151 (2021-10-06)
Expansion microscopy (ExM) enables super-resolution fluorescence imaging on standard microscopes by physical expansion of the sample. However, the investigation of interactions between different organisms such as mammalian and fungal cells by ExM remains challenging because different cell types require different
Polymerization of sodium acrylate in inverse-suspension stabilized by sorbitan fatty esters
Omidian H, et al.
European Polymer Journal, 39(5), 1013-1018 (2003)
N Sahabandu et al.
Journal of microscopy, 276(3), 145-159 (2019-11-07)
Centrioles are vital cellular structures that organise centrosomes and cilia. Due to their subresolutional size, centriole ultrastructural features have been traditionally analysed by electron microscopy. Here we present an adaptation of magnified analysis of the proteome expansion microscopy method, to
Jui-Yang Lai
Materials science & engineering. C, Materials for biological applications, 33(7), 3704-3710 (2013-08-06)
Characterization of corneal cellular response to hydrogel materials is an important issue in ophthalmic applications. In this study, we aimed to investigate the relationship between the feed composition of 2-hydroxyethyl methacrylate (HEMA)/acrylic acid (AAc) and material compatibility towards corneal stromal
Peter Jan Hooikaas et al.
eLife, 9 (2020-12-22)
When a T cell and an antigen-presenting cell form an immunological synapse, rapid dynein-driven translocation of the centrosome toward the contact site leads to reorganization of microtubules and associated organelles. Currently, little is known about how the regulation of microtubule
Eugene A Katrukha et al.
eLife, 10 (2021-07-28)
The neuronal microtubule cytoskeleton underlies the polarization and proper functioning of neurons, amongst others by providing tracks for motor proteins that drive intracellular transport. Different subsets of neuronal microtubules, varying in composition, stability, and motor preference, are known to exist
Gerlinde Lefever et al.
Materials (Basel, Switzerland), 13(22) (2020-11-21)
Superabsorbent polymers (SAPs) are known to mitigate the development of autogenous shrinkage in cementitious mixtures with a low water-to-cement ratio. Moreover, the addition of SAPs promotes the self-healing ability of cracks. A drawback of using SAPs lies in the formation
Changyang Linghu et al.
Cell, 183(6), 1682-1698 (2020-11-25)
In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of
Tyler J Chozinski et al.
Scientific reports, 8(1), 10396-10396 (2018-07-12)
Although light microscopy is a powerful tool for the assessment of kidney physiology and pathology, it has traditionally been unable to resolve structures separated by less than the ~250 nm diffraction limit of visible light. Here, we report on the optimization
Circadian Modulation of Neurons and Astrocytes Controls Synaptic Plasticity in Hippocampal Area CA1.
John P McCauley et al.
Cell reports, 33(2), 108255-108255 (2020-10-15)
Most animal species operate according to a 24-h period set by the suprachiasmatic nucleus (SCN) of the hypothalamus. The rhythmic activity of the SCN modulates hippocampal-dependent memory, but the molecular and cellular mechanisms that account for this effect remain largely
Zhenlu Zhao et al.
Mikrochimica acta, 186(4), 226-226 (2019-03-09)
The authors have fabricated reduced graphene oxide nanosheets (rGO) supported with Fe3O4 nanoparticles and Ag/Au hollow nanoshells. The material was placed on a glassy carbon electrode which is shown to enable highly sensitive determination of As(III) which is first preconcentrated
Antoine Gaudreau-Lapierre et al.
STAR protocols, 2(3), 100630-100630 (2021-07-06)
Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold
Ralph Götz et al.
Nature communications, 11(1), 6173-6173 (2020-12-04)
Expansion microscopy (ExM) enables super-resolution imaging of proteins and nucleic acids on conventional microscopes. However, imaging of details of the organization of lipid bilayers by light microscopy remains challenging. We introduce an unnatural short-chain azide- and amino-modified sphingolipid ceramide, which
Dionis Minev et al.
Nucleic acids research, 47(22), 11956-11962 (2019-11-13)
There is increasing demand for single-stranded DNA (ssDNA) of lengths >200 nucleotides (nt) in synthetic biology, biological imaging and bionanotechnology. Existing methods to produce high-purity long ssDNA face limitations in scalability, complexity of protocol steps and/or yield. We present a
Dongfang Wang et al.
ACS applied materials & interfaces, 10(21), 18316-18326 (2018-05-08)
In this work, a novel nanosystem with a sandwich-like structure was synthesized via face-to-face combination of two pieces of waste cotton fabrics (CFs) carrying ferrous sulfide (FeS) and carboxyl-functionalized ferroferric oxide microsphere (CFFM), respectively, and the obtained nanosystem was named
Sinem K Saka et al.
Nature biotechnology, 37(9), 1080-1090 (2019-08-21)
Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity and throughput. Here we report immunostaining with signal amplification by exchange reaction (Immuno-SABER), which achieves highly multiplexed signal amplification via DNA-barcoded antibodies and orthogonal DNA concatemers generated
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