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Showing 1-30 of 137 results for "408727" within Papers
Martin Fogtmann Berthelsen et al.
Cancers, 13(12) (2021-07-03)
The generation of large transgenic animals is impeded by complex cloning, long maturation and gastrulation times. An introduction of multiple gene alterations increases the complexity. We have cloned a transgenic Cas9 minipig to introduce multiple mutations by CRISPR in somatic
Jessel, N., et al.
Polym. Mater. Sci. Eng., 90, 755-755 (2004)
Helena Sork et al.
Cells, 10(6) (2021-07-03)
The extracellular environment consists of a plethora of molecules, including extracellular miRNA that can be secreted in association with extracellular vesicles (EVs) or soluble protein complexes (non-EVs). Yet, interest in therapeutic short RNA carriers lies mainly in EVs, the vehicles
Elena Britti et al.
The Biochemical journal, 478(1), 1-20 (2020-12-12)
Friedreich ataxia (FA) is a neurodegenerative disease caused by the deficiency of frataxin, a mitochondrial protein. In primary cultures of dorsal root ganglia neurons, we showed that frataxin depletion resulted in decreased levels of the mitochondrial calcium exchanger NCLX, neurite
Claire Ann Butler et al.
Journal of neurochemistry, 158(2), 297-310 (2021-03-16)
CD33 is a Siglec (sialic acid-binding immunoglobulin-type lectin) receptor on microglia. Human CD33 can be alternatively spliced into two isoforms: the long isoform (CD33M) and a shorter isoform (CD33m) that lacks the sialic acid-binding site. CD33m appears to protect against
Jiandong Huo et al.
Cell host & microbe, 28(3), 445-454 (2020-06-26)
There are as yet no licensed therapeutics for the COVID-19 pandemic. The causal coronavirus (SARS-CoV-2) binds host cells via a trimeric spike whose receptor binding domain (RBD) recognizes angiotensin-converting enzyme 2, initiating conformational changes that drive membrane fusion. We find
Hongxu Xian et al.
Autophagy, 15(12), 2107-2125 (2019-03-22)
Mitochondrial dynamics is highly implicated in a plethora of cellular processes including apoptosis and mitophagy. However, little is known about the scope and precise functions of mitochondrial dynamics proteins for mitochondrial quality control and cellular homeostasis. Whether mitochondrial dynamics proteins
Julian Deppe et al.
Toxins, 12(12) (2020-12-02)
The extraordinarily potent clostridial neurotoxins (CNTs) comprise tetanus neurotoxin (TeNT) and the seven established botulinum neurotoxin serotypes (BoNT/A-G). They are composed of four structurally independent domains: the roles of the catalytically active light chain, the translocation domain HN, and the
Q Tu et al.
Oncogene, 37(1), 128-138 (2017-09-12)
Pancreatic cancer is among the deadliest malignancies; however, the genetic events that lead to pancreatic carcinogenesis in adults remain unclear. In vivo models in which these genetic alterations occur in adult animals may more accurately reflect the features of human
Yongquan Hu et al.
Autophagy, 17(5), 1142-1156 (2020-04-07)
Energy deprivation activates the cellular energy sensor AMP-activated protein kinase (AMPK), which in turn induces macroautophagy/autophagy. The mitochondrial-associated ER membrane (MAM) plays a key role in mitochondrial division and autophagy, and the mitochondrial fusion protein MFN2 (mitofusin 2) tethers the
Jo Waaler et al.
Communications biology, 3(1), 196-196 (2020-04-26)
The development of immune checkpoint inhibitors represents a major breakthrough in cancer therapy. Nevertheless, a substantial number of patients fail to respond to checkpoint pathway blockade. Evidence for WNT/β-catenin signaling-mediated immune evasion is found in a subset of cancers including
Xiaofei Xin et al.
Theranostics, 8(13), 3474-3489 (2018-07-22)
Protein therapeutics is playing an increasingly critical role in treatment of human diseases. However, current vectors are captured by the digestive endo-lysosomal system, which results in an extremely low fraction (<2%) of protein being released in the cytoplasm. This paper
Thais Canassa-DeLeo et al.
Glycoconjugate journal, 37(1), 77-93 (2019-12-12)
Dystroglycanopathies are diseases characterized by progressive muscular degeneration and impairment of patient's quality of life. They are associated with altered glycosylation of the dystrophin-glycoprotein (DGC) complex components, such as α-dystroglycan (α-DG), fundamental in the structural and functional stability of the
Edwin J Vazquez-Cintron et al.
Scientific reports, 7, 42923-42923 (2017-02-22)
Botulinum neurotoxin (BoNT) binds to and internalizes its light chain into presynaptic compartments with exquisite specificity. While the native toxin is extremely lethal, bioengineering of BoNT has the potential to eliminate toxicity without disrupting neuron-specific targeting, thereby creating a molecular
Neha Nagpal et al.
Cell stem cell, 26(6), 896-909 (2020-04-23)
Genetic lesions that reduce telomerase activity inhibit stem cell replication and cause a range of incurable diseases, including dyskeratosis congenita (DC) and pulmonary fibrosis (PF). Modalities to restore telomerase in stem cells throughout the body remain unclear. Here, we describe
Li-Ting Wang et al.
Nature communications, 12(1), 4697-4697 (2021-08-06)
Polarized epithelial cells can organize into complex structures with a characteristic central lumen. Lumen formation requires that cells coordinately orient their polarity axis so that the basolateral domain is on the outside and apical domain inside epithelial structures. Here we
Jingtang Li et al.
Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 29(4), 431-440 (2020-05-05)
Autogenous or allogenic bone transplantation is the main treatment for bone defects and nonunions. However, the shortcomings of autogenous or allogenic bone transplantation limit its wide application in clinical use. This study investigated the effect of poly(lactic-co-glycolic acid) (PLGA) microspheres
Naixia Ren et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Functional characterization of cancer risk-associated single nucleotide polymorphism (SNP) identified by genome-wide association studies (GWAS) has become a big challenge. To identify the regulatory risk SNPs that can lead to transcriptional misregulation, we performed parallel reporter gene assays with both
Linlin Z Fan et al.
Nature methods, 15(10), 823-831 (2018-10-03)
Optical assays of synaptic strength could facilitate studies of neuronal transmission and its dysregulation in disease. Here we introduce a genetic toolbox for all-optical interrogation of synaptic electrophysiology (synOptopatch) via mutually exclusive expression of a channelrhodopsin actuator and an archaerhodopsin-derived
Yiting Wang et al.
Cell communication and signaling : CCS, 18(1), 116-116 (2020-07-30)
Aberrant activation of the Hedgehog (Hh) signaling pathway is frequently observed in hepatocellular carcinoma (HCC), nevertheless, the precise molecular mechanism remains unclear. Forkhead box M1 (FOXM1), a target of the Hh pathway, is a key oncofetal transcription factor and a
Josef Novák et al.
Cytokine, 133, 155141-155141 (2020-07-03)
Interleukin-1α (IL-1α) and Annexin A2 (AnxA2) are pleiotropic molecules with both intracellular and extracellular roles. They share several characteristics including unconventional secretion aided by S100 proteins, anchoring of the externalized proteins at the outer surface of the plasma membrane and
David R Wilson et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(7), 1697-1709 (2017-05-10)
There is a need for new tools to better quantify intracellular delivery barriers in high-throughput and high-content ways. Here, we synthesized a triple-fluorophore-labeled nucleic acid pH nanosensor for measuring intracellular pH of exogenous DNA at specific time points in a
Jiyoung Kim et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(24), 13529-13540 (2020-06-03)
The Hippo pathway plays a pivotal role in tissue homeostasis and tumor suppression. YAP and TAZ are downstream effectors of the Hippo pathway, and their activities are tightly suppressed by phosphorylation-dependent cytoplasmic retention. However, the molecular mechanisms governing YAP/TAZ nuclear
Reto Eggenschwiler et al.
Scientific reports, 11(1), 22154-22154 (2021-11-14)
CRISPR prime-editors are emergent tools for genome editing and offer a versatile alternative approach to HDR-based genome engineering or DNA base-editors. However, sufficient prime-editor expression levels and availability of optimized transfection protocols may affect editing efficiencies, especially in hard-to-transfect cells
Mohammad Al Sorkhy et al.
Cell cycle (Georgetown, Tex.), 15(1), 128-136 (2016-01-16)
Families of cyclin-like proteins have emerged that bind and activate cyclin dependent kinases (Cdk)s, directing the phosphorylation of noncanonical Cdk substrates. One of these proteins, Spy1, has demonstrated the unique ability to directly bind and activate both Cdk1 and Cdk2
Yoon-Gu Jang et al.
Molecules and cells, 43(8), 705-717 (2020-08-08)
While the growth factors like insulin initiate a signaling cascade to induce conformational changes in the mechanistic target of rapamycin complex 1 (mTORC1), amino acids cause the complex to localize to the site of activation, the lysosome. The precise mechanism
Patrick M McNutt et al.
Science translational medicine, 13(575) (2021-01-08)
Botulism is caused by a potent neurotoxin that blocks neuromuscular transmission, resulting in death by asphyxiation. Currently, the therapeutic options are limited and there is no antidote. Here, we harness the structural and trafficking properties of an atoxic derivative of
Roberta Risoluti et al.
Journal of pharmaceutical analysis, 10(4), 351-355 (2020-09-15)
In this work, functionalized carbon nanotubes (CNTs) using two polyamine polymers, polyethyleneimine (PEI) and polyamidoamine dendrimer (PAMAM), were investigated by thermal analysis in order to address preparation strategies to obtain low cytotoxic compounds with the ability to conjugate microRNAs and
Yanqiong Chen et al.
Heliyon, 6(8), e04793-e04793 (2020-09-02)
The -1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to
Mireia Nàger et al.
Autophagy, 14(4), 619-636 (2018-01-10)
WNT-CTNN1B signaling promotes cancer cell proliferation and stemness. Furthermore, recent evidence indicates that macroautophagy/autophagy regulates WNT signaling. Here we investigated the impact of inhibiting WNT signaling on autophagy in glioblastoma (GBM), a devastating brain tumor. Inhibiting TCF, or silencing TCF4
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