amidating enzyme and acyl-coenzyme A: glycine
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430-434 (1996).
3. Cravatt, B.F., et. al. Chemical characterization of a
family of brain lipids that induce
may be stored up to 3 months at −20 °C and up to
24 hours at 2−8 °C.
References
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2. He, B. et al., Proc. Natl. Acad. USA, 88, 11373-
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117-120 (1991).
3. Durand-Arczynska, W., et al.,
Histochemistry, 100, 465-471 (1993).
4. van Royen, N., et al., FASEB, 16,
432-434 (2002
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6. Emery, J.G., et al., Osteoprotegerin is a receptor
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CELL LYSATE SAMPLE PREPARATION
1. Culture cells to approximately 85-90% confluency, stimulating Ras activation as desired.
2. Remove culture media and wash cells twice ice-cold with 1X TBS (Tris Buffered
T, Miyakura Y, Horie H, Yasuda Y, Fujii H, Kotake K, Sugano K (2013)
Biochem Biophys Res Commun. 434(4):753-9.
PMID:23583407
Application of Scepter™ Cell Counter: Cell diameter measurement
used as a
encoding splice variant 6 of EP3
(Accession Number: NM_198716)
Cells are frozen at 2 x 106 cells/mL in 90% fetal bovine serum/10% DMSO. Cell line tests
negative for mycoplasma.
STORAGE
Bednarek, M.A. Derivatives of S-9-Fluorenylmethyl-L-cysteine. Int. J. Pept. Protein Res. 1982, 20, 434.
12 Bodznszky, M.; Martinez, J.; Priestley, G.P.; Gardner, J.D.; Mutt, V. Cholecystokinin (Pancreozymin
2007 Millipore Corporation, Billerica, MA 01821 All rights reserved.
hKv3.3 Sequence:
Bases 434-2274 of sequence of the cDNA used to make this cell line correspond
to the accession number NM_004977
ZIX7005T0 100 30 434
ZIX7010T0 200 40 478
ZIX7015T0 300 60 518
Water consumption
Regardless of the water system type, 3 L of feed water are needed to produce 1 L of type 2 purified
water.
kinase activity, respectively). Inhibits c-kit
receptor only at much higher concentrations (IC50 = 434 and
234 nM in receptor phosphorylation and kinase activity,
respectively). Purity: ≥95% by HPLC.
for 2 hours and subsequently washed prior to co-culture with CAR T
cells at different ratios for 4 hours. Co-culture supernatants were mixed with Optiphase super-
mix (PerkinElmer, cat no 1200–434) and