stage-specific embryonic antigens (SSEA-1, 3 and 4), tumor rejection antigens
(TRA-1-60 and TRA-1-81), Oct-4 and alkaline phosphatase. These undifferentiated markers are
downregulated upon spontaneous
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Corporation, 28820 Single Oak Drive, Temecula, CA 92590, USA 1-800-437-7500
Technical Support: T: 1-800-MILLIPORE (1-800-645-5476) • F: 1-800-437-7502
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures
Not for use in diagnostic procedures
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alkaline phosphatase, expressed the correct human pluripotent markers (Oct4,
SSEA-4, TRA-1-60 and TRA-1-81) and can be expanded in normal human ES cell culture conditions.
The use of L-Myc in the context
Prepare 90 mL of 1x Enrichment Buffer by adding 9 mL of 10x
Enrichment Buffer (Part No. CS210560) to 81 mL of Milli-Q or deionized water. Store at 2-8ºC.
3. 1x Assay Buffer: Prepare
markers including alkaline phosphatase (B), SSEA-4 (C), Oct-4 (D), SSEA-3 (E), TRA-1-60 (F) and
TRA-1-81 (G). Human iPS cells do not express SSEA-1 (data not shown).
9
References
express human pluripotent
markers including alkaline phosphatase (B), SSEA-4 (C), Oct-4 (D), TRA-1-81(E), and SSEA-3 (F) Human iPS
cells do not express SSEA-1 (G).
9
References
1.
pluripotent markers including alkaline phosphatase (A), Oct-4 (B),
SSEA-4 (C), TRA-1-60 (D) and TRA-1-81 (E). Human iPS cells do not express SSEA-1 (F).