Reagent Preparation
Prepare 1x Wash buffer by adding the contents of the
bottle (25 mL, 20x) to 475 mL of distilled or deionized
water. Store at room temperature (18–26 °C).
2
Storage/
C.D., et al., BMP-6 is an autocrine
stimulator of chondrocyte differentiation. Bone Min.
Res., 14, 475-482 (1999).
10. Knittel, T., et al., Bone morphogenetic protein-6 is
expressed in nonparenchymal
C.D., et al., BMP-6 is an autocrine
stimulator of chondrocyte differentiation. Bone Min.
Res., 14, 475-482 (1999).
10. Knittel, T., et al., Bone morphogenetic protein-6 is
expressed in nonparenchymal
least
4 months.
2. Prepare 1x Wash buffer by adding the contents of
the bottle (25 mL, 20x) to 475 mL of distilled or
deionized water. Store at room temperature
(18–26 °C).
Storage/Stability
Store
day.
20x Wash Buffer Concentrate
Prepare 1x wash buffer by adding the contents of the
bottle to 475 mL of distilled water. Store 1x Wash
buffer at room temperature.
Storage/Stability
Store the kit
2
20× Wash Buffer Concentrate
Prepare 1× wash buffer by adding the contents of the
bottle to 475 ml of distilled water. Store 1× wash buffer
at room temperature.
20× Enzyme Conjugate
Prepare 1
day.
20x Wash Buffer Concentrate
Prepare 1x wash buffer by adding the contents of the
bottle to 475 mL of distilled water. Store 1x Wash
buffer at room temperature.
Storage/Stability
Store the kit
day.
20x Wash Buffer Concentrate
Prepare 1x wash buffer by adding the contents of the
bottle to 475 mL of distilled water. Store 1x Wash buffer
at room temperature.
Storage/Stability
Store the kit
25 µL of the
intermediate stock into the 475 µL aliquot of each assay buffer (refer to Table 4
for Detection Antibody preparation layout).
1 2 3 4 5 6 7 8 9 10 11 12
25 µL of the
intermediate stock into the 475 µL aliquot of each assay buffer (refer to Table 4
for Detection Antibody preparation layout).
1 2 3 4 5 6 7 8 9 10 11 12
(2004).
32. Robyr, D., et al., Cell, 109, 437-446 (2002).
33. Narlikar, G.J., et al., Cell, 108, 475-487 (2002).
34. Jepsen, K. and Rosenfeld, M.G., J. Cell. Sci., 115, 689-698 (2002).
35. Bowen, N.
intermediate detection antibody stock 20-fold by adding 25 μL of the
intermediate stock into the 475 μL aliquot of each optimization assay buffer in the
deep-well plate.
a. Refer to Figure 12 for
Wash fractions 7 to 20 (pool)
Lanes 10 to 19: Elution fractions 1 to 10
Lane 20: Original in vitro expression and biotinylation mixture
Lanes 21 to 25: CAT standards 158 ng, 317 ng, 475 ng, 633 ng, and
resolution of human cerebrospinal fl uid proteins on two-di-
mensional gels. Multiple Sclerosis 2003, 9:472-475.
120. Li C, Lee KH: Affi nity depletion of albumin from human cere-
brospinal fl uid using Cibacron-blue
human hepatoma
HepaRG™ cells under various experimental conditions.
Toxicol In Vitro 23, 466-475 (2009).
38. McGill MR et al.. HepaRG™ cells: a human model to study
mechanisms of acetaminophen hepatotoxicity
et al. 2004. Int. J. Dev. Neurobiol. 22, 475.
Feng, R., et al. 2004. Proc. Natl. Acad. Sci. USA 101, 8162.
Gibson, G. E., and Huang, H. M. 2002, Front. Biosci. 7, d1007.
Sisodia, S.S., and St George-Hyslop
bandwidth. For
example, an excitation bandwidth for fluorescein could be set at 485 nm +/- 10 nm (i.e.,
475 to 495 nm) and an emission of 535 nm +/- 15 nm (520 to 550 nm). Wider windows
have a greater
bandwidth. For
example, an excitation bandwidth for fluorescein could be set at 485 nm +/- 10 nm (i.e.,
475 to 495 nm) and an emission of 535 nm +/- 15 nm (520 to 550 nm). Wider windows
have a greater
bandwidth. For
example, an excitation bandwidth for fluorescein could be set at 485 nm +/- 10 nm (i.e.,
475 to 495 nm) and an emission of 535 nm +/- 15 nm (520 to 550 nm). Wider windows
have a greater
described in the assay instructions.
Description Catalogue No.
Phospho-p53 (Ser15) STAR ELISA Kit 17-475
Anti-Chk1 04-207
Anti-Plk1 05-844
Anti-Wee1 06-972
p53 STAR ELISA Kit
In response to DNA