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Showing 1-30 of 36 results for "487928" within Papers
David W Sanders et al.
eLife, 10 (2021-04-24)
Many enveloped viruses induce multinucleated cells (syncytia), reflective of membrane fusion events caused by the same machinery that underlies viral entry. These syncytia are thought to facilitate replication and evasion of the host immune response. Here, we report that co-culture
Hieng Chiong Tie et al.
Methods in molecular biology (Clifton, N.J.), 2557, 113-125 (2022-12-14)
The Golgi complex is essential for protein transport and posttranslational modification in mammalian cells. It is critical to know the cisternal distribution of Golgi proteins to understand Golgi functions. The cis-to-trans or axial localization of a Golgi protein can be
Alexandre Santinho et al.
Nature communications, 15(1), 3767-3767 (2024-05-05)
Tools for accessing and studying organelles remain underdeveloped. Here, we present a method by which giant organelle vesicles (GOVs) are generated by submitting cells to a hypotonic medium followed by plasma membrane breakage. By this means, GOVs ranging from 3
Stephen DiGiuseppe et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(22), 6289-6294 (2016-05-18)
During the entry process, the human papillomavirus (HPV) capsid is trafficked to the trans-Golgi network (TGN), whereupon it enters the nucleus during mitosis. We previously demonstrated that the minor capsid protein L2 assumes a transmembranous conformation in the TGN. Here
Nicolás Cifuentes-Muñoz et al.
Journal of virology, 91(24) (2017-10-06)
Human metapneumovirus (HMPV) causes significant upper and lower respiratory disease in all age groups worldwide. The virus possesses a negative-sense single-stranded RNA genome of approximately 13.3 kb encapsidated by multiple copies of the nucleoprotein (N), giving rise to helical nucleocapsids.
Joaquim Javary et al.
Journal of cellular physiology, 239(3), e30938-e30938 (2022-12-29)
Extracellular signal-regulated kinase 3 (ERK3) is a poorly characterized member of the mitogen-activated protein (MAP) kinase family. Functional analysis of the ERK3 signaling pathway has been hampered by a lack of knowledge about the substrates and downstream effectors of the
Aleksandar I Mihajlović et al.
EMBO reports, 24(11), e57227-e57227 (2023-10-05)
Chromosome segregation errors in mammalian oocyte meiosis lead to developmentally compromised aneuploid embryos and become more common with advancing maternal age. Known contributors include age-related chromosome cohesion loss and spindle assembly checkpoint (SAC) fallibility in meiosis-I. But how effective the
Sílvia Vale-Costa et al.
PLoS biology, 21(11), e3002290-e3002290 (2023-11-20)
It is now established that many viruses that threaten public health establish condensates via phase transitions to complete their lifecycles, and knowledge on such processes may offer new strategies for antiviral therapy. In the case of influenza A virus (IAV)
Helena Vandesande et al.
Journal of virology, 94(13) (2020-04-17)
Echovirus 30 (E30), a member of the enterovirus B species, is a major cause of viral meningitis, targeting children and adults alike. While it is a frequently isolated enterovirus and the cause of several outbreaks all over the world, surprisingly
Toshiyuki Habu et al.
STAR protocols, 4(1), 102063-102063 (2023-03-01)
Here, we present a protocol combining co-immunoprecipitation (Co-IP) and immunofluorescence approaches with cell cycle stage synchronization to detect cell-cycle-specific complexes. We describe steps to synchronize cells at specific cell cycle stages using drugs. We then detail the preparation of cell
Masashi Minamino et al.
Current biology : CB, 28(16), 2665-2672 (2018-08-14)
Sister chromatid cohesion, mediated by cohesin, is required for accurate chromosome segregation [1, 2]. This process requires acetylation of cohesin subunit SMC3 by evolutionarily conserved cohesin acetyltransferases: Eco1 in budding yeast; XEco1 and XEco2 in Xenopus; and ESCO1 and ESCO2 in
Matthias Weiβ et al.
Cell reports, 42(2), 112045-112045 (2023-01-27)
The chromatin environment at origins of replication is thought to influence DNA replication initiation in eukaryotic genomes. However, it remains unclear how and which chromatin features control the firing of early-efficient (EE) or late-inefficient (LI) origins. Here, we use site-specific
Tsuyoshi Hirashima et al.
Current biology : CB, 34(4), 683-696 (2024-01-17)
Intricate branching patterns emerge in internal organs due to the recurrent occurrence of simple deformations in epithelial tissues. During murine lung development, epithelial cells in distal tips of the single tube require fibroblast growth factor (FGF) signals emanating from their
Steffen Israel et al.
Molecular human reproduction, 29(11) (2023-11-06)
In oocyte biology, the zona pellucida has long been known to operate three extracellular functions downstream of the secretory pathway, namely, encasing the oocytes in ovarian follicles, mediating sperm-oocyte interaction, and preventing premature embryo contact with oviductal epithelium. The present
Cécile Jebane et al.
iScience, 26(10), 107714-107714 (2023-09-13)
Lamin A/C is a well-established key contributor to nuclear stiffness and its role in nucleus mechanical properties has been extensively studied. However, its impact on whole-cell mechanics has been poorly addressed, particularly concerning measurable physical parameters. In this study, we
Visualizing the Cisternal Organization of Golgi Ministacks in HeLa Cells by Side-averaging.
Mahajan, et al.
Bio-protocol, 13, e4658-e4658 (2023)
Esra Unsal et al.
Chemical biology & drug design, 88(6), 783-794 (2016-11-05)
Aurora B is a serine/threonine kinase that has a central role in the regulation of mitosis. The observation of Aurora B overexpression in cancer makes it a promising target to develop antitumoral inhibitors. We describe a new potential inhibitor that
Benoit Dehapiot et al.
PLoS biology, 19(9), e3001376-e3001376 (2021-09-08)
Mammalian oocyte meiotic divisions are highly asymmetric and produce a large haploid gamete and 2 small polar bodies. This relies on the ability of the cell to break symmetry and position its spindle close to the cortex before anaphase occurs.
Spencer A Freeman et al.
Science (New York, N.Y.), 367(6475), 301-305 (2019-12-07)
Despite ongoing (macro)pinocytosis of extracellular fluid, the volume of the endocytic pathway remains unchanged. To investigate the underlying mechanism, we used high-resolution video imaging to analyze the fate of macropinosomes formed by macrophages in vitro and in situ. Na+, the
Lisa Crozier et al.
The EMBO journal, 41(6), e108599-e108599 (2022-01-18)
CDK4/6 inhibitors arrest the cell cycle in G1-phase. They are approved to treat breast cancer and are also undergoing clinical trials against a range of other tumour types. To facilitate these efforts, it is important to understand why a cytostatic
Hieng Chiong Tie et al.
The Journal of cell biology, 221(6) (2022-04-26)
The mammalian Golgi comprises tightly adjacent and flattened membrane sacs called cisternae. We still do not understand the molecular organization of the Golgi and intra-Golgi transport of cargos. One of the most significant challenges to studying the Golgi is resolving
Yi-Hsuan Ho et al.
Current biology : CB, 28(16), 2673-2680 (2018-08-07)
Stress tolerance and rapid growth are often competing interests in cells. Upon severe environmental stress, many organisms activate defense systems concurrent with growth arrest. There has been debate as to whether aspects of the stress-activated transcriptome are regulated by stress
Zeynep Cansu Uretmen Kagiali et al.
Life science alliance, 3(2) (2019-12-28)
CLIC4 and CLIC1 are members of the well-conserved chloride intracellular channel proteins (CLICs) structurally related to glutathione-S-transferases. Here, we report new roles of CLICs in cytokinesis. At the onset of cytokinesis, CLIC4 accumulates at the cleavage furrow and later localizes
Barbara Gisevius et al.
Brain communications, 6(3), fcae182-fcae182 (2024-06-19)
Neurodegeneration in the autoimmune disease multiple sclerosis still poses a major therapeutic challenge. Effective drugs that target the inflammation can only partially reduce accumulation of neurological deficits and conversion to progressive disease forms. Diet and the associated gut microbiome are
Pei Xuan Song et al.
iScience, 25(5), 104288-104288 (2022-05-17)
The accumulation of lipid droplets (LDs) in the liver is a hallmark of steatosis, which is often associated with lysosomal dysfunction. Nevertheless, the underlying mechanisms remain unclear. Here, using Huh7 cells loaded with oleate as a model to study LD
Clotilde C N Renaud et al.
iScience, 27(4), 109580-109580 (2024-04-11)
Centriolar satellites are high-order assemblies, scaffolded by the protein PCM1, that gravitate as particles around the centrosome and play pivotal roles in fundamental cellular processes notably ciliogenesis and autophagy. Despite stringent control mechanisms involving phosphorylation and ubiquitination, the landscape of
Kozue Hamao et al.
Experimental cell research, 386(1), 111707-111707 (2019-11-07)
Cytokinesis of animal cells requires contraction of a contractile ring, composed of actin filaments and myosin II filaments. Phosphorylation of myosin II regulatory light chain (MRLC) promotes contraction of the actomyosin ring by activating myosin II motor activity. Both Rho-associated
Kinetochore deterioration concommitant with centromere weakening during aging in mouse oocyte meiosis-I.
Yin, et al.
Faseb Journal, 37, e22922-e22922 (2023)
Hao-Yang Liu et al.
Nature protocols (2022-12-01)
Single-virus tracking (SVT) offers the opportunity to monitor the journey of individual viruses in real time and to explore the interactions between viral and cellular structures in live cells, which can assist in characterizing the complex infection process and revealing
Fan Pan et al.
Cell death & disease, 14(7), 430-430 (2023-07-15)
Tumor progression and evolution are frequently associated with chromosomal instability (CIN). Tumor cells often express high levels of the mitotic checkpoint protein MAD2, leading to mitotic arrest and cell death. However, some tumor cells are capable of exiting mitosis and
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