5, No 2, 41 (1989).
3. Imai, T. and Olson, M., Genomics, 8, 297 (1990).
4. Stefano, V., et al., Biochemistry International, 27,
45 (1992).
5. Yaphe, W., Can. J. Microbiol., 3, 987 (1957).
7/
5, No 2, 41 (1989).
3. Imai, T. and Olson, M., Genomics, 8, 297 (1990).
4. Stefano, V., et al., Biochemistry International, 27,
45 (1992).
5. Yaphe, W., Can. J. Microbiol., 3, 987 (1957).
1/
5, No 2, 41 (1989).
3. Imai, T. and Olson, M., Genomics, 8, 297 (1990).
4. Stefano, V., et al., Biochemistry International, 27,
45 (1992).
5. Yaphe, W., Can. J. Microbiol., 3, 987 (1957).
8/
5, No 2, 41 (1989).
3. Imai, T. and Olson, M., Genomics, 8, 297 (1990).
4. Stefano, V., et al., Biochemistry International, 27,
45 (1992).
5. Yaphe, W., Can. J. Microbiol., 3, 987 (1957).
6/
5, No 2, 41 (1989).
3. Imai, T. and Olson, M., Genomics, 8, 297 (1990).
4. Stefano, V., et al., Biochemistry International, 27,
45 (1992).
5. Yaphe, W., Can. J. Microbiol., 3, 987 (1957).
2/
products >3 kb.
Step Temperature [°C] Time Number of Cycles
Pre-Incubation 94 2 min 1
Denaturation
Annealing
Elongation
94
45 – 65(1)
72(2) or 68
15 sec
30 sec
45 sec – 8 min(3)
10
purified water.
• Add 5 mL of this solution to 1 L of the melted mix (step 1) at 45-50 °C.
• Stir well for homogenization
• Step 3:
• Pour into sterile Petri dishes.
• Let it solidify and
E
N
Indole-3-butyric acid
(4-(3-Indolyl)butyric acid)
CAS No. 133-32-4
C12H13NO2 FW 203.2
Plant cell culture, tested
R: 25-36/37/38 S: 26-37-45
5 g
25 g
I 7512
E
Indole-3-butyric acid potassium
for 50 µL reaction
volumes but can effectively purify samples from
45- 55 µL.
• For PCR volumes > 55 µL, aliquot 50 µL into each
tube for purification.
• For PCR volumes 45 µL, dilute to 50 µL.
of the 250 M L-Lactate and 45 L of
Sample to separate wells of the plate.
3. For the Sample wells (See Sample
Preparation, Step 3b), add 5 L of
purified water and 45 L of Sample
bottle 3) for 10
min at +15 to +25°C.
6.1.4 Immuno-
detection
Sediment cells (300 × g, 10 min) and resuspend pellet
in 50 �l anti-BrdU-FLUOS antibody working solution
(II). Incubate for 45 min at
Elongation time 45 sec 1 min 2 min 3 min 4 min
Temperature for RT-step [°C] ≤60 50 50 48 45 – 48
(3) The cycle number is dependent on the abundance of the respective mRNA. For rare messages
water bath at 45-50 °C, swirling or stirring gently.
Advice 2: In case of samples with a high presence of Aeromonas, 50 mg of cefsulodin can be added to
the mix once cooled down at 45-50 °C (50 mg/L).